Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat

• Published in: Theoretical and applied genetics Vol. 126; no. 2; pp. 523 - 533
• Main Authors: Xu, L. S, Wang, M. N, Cheng, P, Kang, Z. S, Hulbert, S. H, Chen, X. M
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.02.2013; Springer; Springer Nature B.V
• Subjects: Agriculture; Basidiomycota; Basidiomycota - pathogenicity; Basidiomycota - physiology; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chromosome Mapping; chromosomes; Chromosomes, Plant; Chromosomes, Plant - genetics; Cultivars; Disease; Disease Resistance; Disease Resistance - genetics; Diseases and pests; dominant genes; durum wheat; Ethiopia; Gene expression; Genes; Genes, Plant; Genes, Plant - genetics; Genetic aspects; Genetic Linkage; Genetic Linkage - genetics; genetics; Genomes; genotype; Immunity, Innate; immunology; Infections; Life Sciences; microbiology; microsatellite repeats; Molecular genetics; Original Paper; pathogenicity; Phenotype; Physiological aspects; physiology; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Genetics and Genomics; Plant immunology; Plant resistance; Puccinia striiformis; races; Rust diseases; seedlings; Seeds; Triticum; Triticum - genetics; Triticum - immunology; Triticum - microbiology; Triticum aestivum; United States; Wheat; United States; Ethiopia; United States > US; Durum Wheat; Stripe Rust; Common Wheat; Simple Sequence Repeat Marker; Wheat Genotype
• ISSN: 0040-5752; 1432-2242; 1432-2242
• DOI: 10.1007/s00122-012-1998-0

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most damaging diseases of wheat worldwide. It is essential to identify new genes for effective resistance against the disease. Durum wheat PI 480148, originally from Ethiopia, was resistant in all seedling tests with several predominant Pst races in the US under controlled greenhouse conditions and at multiple locations subject to natural infection for several years. To map the resistance gene(s) and to transfer it to common wheat, a cross was made between PI 480148 and susceptible common wheat genotype Avocet S (AvS). Resistant F₃ plants with 42 chromosomes were selected cytologically and by testing with Pst race PST-100. A total of 157 F₄ plants from a single F₃ plant with 2n = 42 tested with PST-100 segregated in a 3 resistant: 1 susceptible ratio, indicating that a single dominant gene from PI 480148 conferred resistance. Using the F₃:₄ population and the resistance gene-analog polymorphism (RGAP) and simple sequence repeat (SSR) markers, the gene was mapped to the long arm of chromosome 2B. SSR marker Xwmc441 and RGAP marker XLRRrev/NLRRrev ₃₅₀ flanked the resistance gene by 5.6 and 2.7 cM, respectively. The effective resistance of the gene to an Australian Pst isolate virulent to Yr5, which is also located on 2BL and confers resistance to all US Pst races, together with an allelism test of the two genes, indicated that the gene from PI 480148 is different from Yr5 and should be a new and useful gene for resistance to stripe rust. Resistant common wheat lines with plant types similar to AvS were selected for use in breeding programs.