同位素稀释-激光剥蚀-电感耦合等离子体质谱法测定生物组织样品中铁元素的含量

针对目前采用同位素稀释-激光剥蚀-电感耦合等离子体质谱（ID-LA-ICP-MS）对固体生物组织切片样品难以实现原位准确定量的难题,本研究将同位素稀释法与LA-ICP-MS技术相结合,通过开展生物组织样品与浓缩稀释剂的同位素充分交换平衡、稀释剂添加方式、原位的同位素比测量等关键技术研究,确定了组织切片与同位素稀释剂的最佳平衡时间、稀释剂的质量以及选用甲醇作为稀释剂溶剂等实验条件,建立了基于同位素稀释技术的LA-ICP-MS技术在生物样品组织切片中Fe元素的微区定量分析方法,并采用实验室自行制备的均匀的山羊脑和牛肝组织切片标准样品对方法进行了验证,通过ID-LA-ICP-MS方法的测量结果与微波...

Full description

Saved in:
Bibliographic Details
Published in分析化学 Vol. 42; no. 4; pp. 536 - 541
Main Author 冯流星 王军
Format Journal Article
LanguageChinese
Published 中国计量科学研究院化学计量与分析科学研究所,北京,100013 2014
Subjects
原位微区定量
同位素稀释
激光剥蚀电感耦合等离子体质谱
组织切片
原位微区定量
Tissue Section
组织切片
Isotope dilution
Laser ablation-inductively coupled plasma-mass spectrometry
同位素稀释
In-situ quantification
激光剥蚀电感耦合等离子体质谱
Online AccessGet full text
ISSN0253-3820
DOI10.3724/SP.J.1096.2014.31003

Cover

More Information
Summary:针对目前采用同位素稀释-激光剥蚀-电感耦合等离子体质谱（ID-LA-ICP-MS）对固体生物组织切片样品难以实现原位准确定量的难题,本研究将同位素稀释法与LA-ICP-MS技术相结合,通过开展生物组织样品与浓缩稀释剂的同位素充分交换平衡、稀释剂添加方式、原位的同位素比测量等关键技术研究,确定了组织切片与同位素稀释剂的最佳平衡时间、稀释剂的质量以及选用甲醇作为稀释剂溶剂等实验条件,建立了基于同位素稀释技术的LA-ICP-MS技术在生物样品组织切片中Fe元素的微区定量分析方法,并采用实验室自行制备的均匀的山羊脑和牛肝组织切片标准样品对方法进行了验证,通过ID-LA-ICP-MS方法的测量结果与微波消解-同位素稀释方法的测量结果相一致,验证了该方法的有效性和可靠性.本方法可进一步应用于临床中生物组织切片样品中金属元素的原位、微区定量测量及成像分析.
Bibliography:FENG Liu-Xing , WANG Jun （ National Institute of Metrology, Belting 100013, China）
Keywords Isotope dilution; Laser ablation-inductively coupled plasma-mass spectrometry; In-situquantification ; Tissue Section
22-1125/O6
Abstract Traceable methods for the determination of total metal concentrations, as well as the regional spatial distribution （quantitative imaging） in biological tissues, are important to understand the pathogenesis and potential treatment of neurodegenerative diseases. Calibration strategies for quantitative analysis of trace elements in biological tissues sections have been reported earlier. However, their validation still remains a challenge due to the lack of suitable certified reference materials and primary methods for quantitative elemental analysis. In this work, a methodology based on isotope dilution calibration with laser ablation （LA） - ICP-MS is, for the first time, developed for the accurate quantitative analysis of iron in thin sections of biological tissue. A novel approach was d
ISSN:0253-3820
DOI:10.3724/SP.J.1096.2014.31003