牛血清白蛋白-三聚氰胺偶联物修饰的表面等离子体激元共振芯片检测三聚氰胺含量

基于竞争反应模式,建立了表面等离子体激元共振（ Surface plasmon resonance, SPR）技术检测三聚氰胺的方法。首先在羧甲基葡聚糖修饰的芯片表面引入牛血清白蛋白-三聚氰胺偶联物（ BSA-melamine）,随后流动注射待测三聚氰胺与适量三聚氰胺单克隆抗体的混合溶液。基于溶液中三聚氰胺与芯片表面固定的BSA-melamine偶联物和溶液中固定浓度的三聚氰胺单克隆抗体之间的竞争反应,通过检测芯片表面结合的抗体所产生的SPR信号,对溶液中三聚氰胺进行定量分析。随着溶液中待测三聚氰胺浓度的增大,SPR信号随之降低。三聚氰胺检测的线性范围为0.25~13 nmol/L及13~25...

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Bibliographic Details
Published in分析化学 Vol. 42; no. 5; pp. 695 - 700
Main Author 刘珊珊 衣馨瑶 王建秀
Format Journal Article
LanguageChinese
Published 中南大学化学化工学院,长沙,410083 2014
Subjects
三聚氰胺
牛血清白蛋白-三聚氰胺偶联物
竞争反应
表面等离子体激元共振
牛血清白蛋白-三聚氰胺偶联物
三聚氰胺
Melamine
Bovine serum albumin-melamine conjugate
竞争反应
Surface plasmon resonance
表面等离子体激元共振
Competitive assay
Online AccessGet full text
ISSN0253-3820
DOI10.3724/SP.J.1096.2014.31197

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Summary:基于竞争反应模式,建立了表面等离子体激元共振（ Surface plasmon resonance, SPR）技术检测三聚氰胺的方法。首先在羧甲基葡聚糖修饰的芯片表面引入牛血清白蛋白-三聚氰胺偶联物（ BSA-melamine）,随后流动注射待测三聚氰胺与适量三聚氰胺单克隆抗体的混合溶液。基于溶液中三聚氰胺与芯片表面固定的BSA-melamine偶联物和溶液中固定浓度的三聚氰胺单克隆抗体之间的竞争反应,通过检测芯片表面结合的抗体所产生的SPR信号,对溶液中三聚氰胺进行定量分析。随着溶液中待测三聚氰胺浓度的增大,SPR信号随之降低。三聚氰胺检测的线性范围为0.25~13 nmol/L及13~250 nmol/L。通过流动注射NaOH溶液可实现芯片的再生,从而大大提高了样品分析通量。本方法解决了SPR技术不易直接检测低浓度小分子的缺陷,具有方便、快捷、成本低等优点。
Bibliography:Surface plasmon resonance;Bovine serum albumin-melamine conjugate;Melamine;Competitive assay
22-1125/O6
LIU Shan-Shan, YI Xin-Yao, WANG Jian-Xiu （College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China）
In this study, surface plasmon resonance （ SPR） assay of melamine was carried out based on a competitive reaction in which the assayed melamine with varying concentrations in solution competed with the pre-immobilized BSA-melamine conjugate for the anti-melamine monoclonal antibody （ mAb） in solution. The BSA-melamine conjugate was first immobilized on the carboxymethylated dextran-covered SPR chips, and this was followed by the injection of the mixed solution of melamine and anti-melamine mAb with a fixed concentration. By examining the SPR signals caused by the attached monoclonal antibodies, the concentration of melamine in solution could be determined. The linear range of the method was 0. 25-13 nmol/L and 13-250 nmol/L. The chips could be regenerated via injection of N
ISSN:0253-3820
DOI:10.3724/SP.J.1096.2014.31197