阴/阳离子交换色谱-电感耦合等离子体质谱法分析鱼和贝类海产品砷的形态

采用阴（Hamilton PRP-X100柱）阳（Dionex Ionpac CS-10柱）离子交换色谱-电感耦合等离子体质谱联用技术,分别以pH 10.3的20mmol/L NH4HCO3和pH 2.0的5mmol/L吡啶溶液为流动相,建立了三价砷As（Ⅲ）、五价砷As（Ⅴ）、一甲基砷酸MMA、二甲基砷酸DMA、砷甜菜碱AsB、砷糖PO4、砷糖OH、砷糖SO3、砷糖SO4砷形态的分析方法。采用微波消解法和超声溶剂提取法对我国不同海域9种鱼和贝类海产品进行前处理,对砷含量及其化学形态进行分析。实验表明,鱼和贝海产品主要砷形态为砷甜菜碱AsB,其含量占可提取砷的42.8%～99.4%,大多数贝类...

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Bibliographic Details
Published in分析化学 Vol. 39; no. 10; pp. 1577 - 1581
Main Author 李卫华 刘玉海
Format Journal Article
LanguageChinese
Published 江苏技术师范学院化学化T学院,常州,213001 2011
Subjects
形态分析
砷
高效液相色谱-电感耦合等离子体质谱
鱼和贝类海产品
鱼和贝类海产品
高效液相色谱-电感耦合等离子体质谱
砷
形态分析
Online AccessGet full text
ISSN0253-3820
DOI10.3724/SP.J.1096.2011.00000

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Summary:采用阴（Hamilton PRP-X100柱）阳（Dionex Ionpac CS-10柱）离子交换色谱-电感耦合等离子体质谱联用技术,分别以pH 10.3的20mmol/L NH4HCO3和pH 2.0的5mmol/L吡啶溶液为流动相,建立了三价砷As（Ⅲ）、五价砷As（Ⅴ）、一甲基砷酸MMA、二甲基砷酸DMA、砷甜菜碱AsB、砷糖PO4、砷糖OH、砷糖SO3、砷糖SO4砷形态的分析方法。采用微波消解法和超声溶剂提取法对我国不同海域9种鱼和贝类海产品进行前处理,对砷含量及其化学形态进行分析。实验表明,鱼和贝海产品主要砷形态为砷甜菜碱AsB,其含量占可提取砷的42.8%～99.4%,大多数贝类样品含有砷糖PO4、砷糖OH,其含量分别占可提取砷的32.5%～57.1%和1.4%～3.3%,少数样品含有DMA,其含量分别占可提取砷的5.7%～25.0%,所有样品无机砷的浓度均低于可提取砷的2.0%。在鱼和贝类海产品中,含砷化合物主要是毒性较低的有机砷,不会对人的健康带来危害。
Bibliography:The separation of arsenic compounds,including As,As,monomethylarsonate（MMA）,dimethylarsonate（DMA）,arsenobetaine（AsB）,arsenosugar PO4,arsenosugar OH,arsenosugar SO3 and arsenosugar SO4 were carried out in Hamilton PRP-X100 anion-exchange column with a mobile phase of 20 mmol/L NH4HCO3（pH 10.3） and on a Dionex Ionpac CS-10 cation-exchange column with a mobile phase of 5 mmol/L pyridinium（pH 2.0） for their analysis by ICP-MS.Total content of As and its different species of eight types of fish and shellfish in microwave-assisted digestion and ultrasonic solvent extraction were examined.The results showed that main components in all of the extracted fish and shellfish samples were arsenobetain（42.8%-99.4%）,while arsenosugar PO4 and arsenosugar OH were detected in most extracted shellfish samples（32.5%-57.1%,1.4%-3.3%）,DMA was detected in a few extracted samples（5.7%-25.0%）.In contrast,no inorganic arsenic in fish and shellfish samples occurred at levels below 2.0%.
High preferment liquid chromatography-mass spect
ISSN:0253-3820
DOI:10.3724/SP.J.1096.2011.00000