分光光度计法测量氨基聚醚的专属性

氨基聚醚（K2.2.2）含量是18F-FDG质控中的关键指标,比较了两种常用测量K2.2.2方法的专属性。分别采用分光光度计和半定量TLC碘显色法测量了14个样品,其中9个阴性样品、2个阳性样品和3个供试品,并与LC-MS/MS测量对比。结果显示：9个阴性样品经分光光度计法测量均为阳性,K2.2.2的测量结果在6.7-470.0μg/mL;2个阳性样品结果偏高（53,73μg/mL）,3个供试品的K2.2.2含量在14.3-19.2μg/mL;半定量TLC碘显色法测量9个阴性样品结果为阴性,2个阳性样品半定量结果与实际一致,3个供试品的K2.2.2含量低于10μg/mL;LC-MS/MS法测量...

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Bibliographic Details
Published in同位素 Vol. 28; no. 3; pp. 129 - 134
Main Author 张锦明 陈征宇 武向峰 张晓军 朱华 周明 杨志
Format Journal Article
LanguageChinese
Published 解放军总医院 核医学科,北京,100853%总后勤部卫生部药品器材局,北京,100842%解放军总后药品检验所,北京,100071%北京肿瘤医院 核医学科,北京,100036 2015
Subjects
TLC
专属性
分光光度计法
氨基聚醚
液质联用
spectrophotometry
LC-MS/MS
液质联用
氨基聚醚
分光光度计法
specificity
TLC
专属性
aminopolyether
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ISSN1000-7512
DOI10.7538/tws.2015.28.03.0129

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Summary:氨基聚醚（K2.2.2）含量是18F-FDG质控中的关键指标,比较了两种常用测量K2.2.2方法的专属性。分别采用分光光度计和半定量TLC碘显色法测量了14个样品,其中9个阴性样品、2个阳性样品和3个供试品,并与LC-MS/MS测量对比。结果显示：9个阴性样品经分光光度计法测量均为阳性,K2.2.2的测量结果在6.7-470.0μg/mL;2个阳性样品结果偏高（53,73μg/mL）,3个供试品的K2.2.2含量在14.3-19.2μg/mL;半定量TLC碘显色法测量9个阴性样品结果为阴性,2个阳性样品半定量结果与实际一致,3个供试品的K2.2.2含量低于10μg/mL;LC-MS/MS法测量的14个样品的结果与半定量TLC碘显色法的结果一致。以上结果表明,半定量TLC碘显色法测量K2.2.2的专属性较好,适用于测量18F-FDG溶液中K2.2.2含量。
Bibliography:11-2566/TL
ZHANG Jin-ming , CHEN Zheng-yu , WU Xiang-feng , ZHANG Xiao-jun, ZHU Hua, ZHOU Ming, YANG Zhi4（1. Dept of Nucl Med , The PLA General Hops pital , Beij ing 100853, China ;2. Drug and Medical Equipment Administration Bureau of Health Department, Beij ing 100842, China 3. Drug and Medical Equipment Inspection Instritue of Health Department, Beijing 100071, China; 4. Dept of Nucl IVied, Peking University Cancer Hospital, Beijing 100036, China）
The The spec 14 s The residue of K2.2.2 was one of the key metric in ^18F-FDG quality control. ificity of the spectrophotometry for K2.2.2 was compared with others. Methods： amples were determined for the K2.2.2 by the spectrophotometry and iodinevapor TLC stain method, respectively. The results were compared with LC-MS/MS. The spectrophotometry showed that the 9 of negative samples were false positive , 2 of positive samples were higher than its actual K2.2.2 contents, 3 of ^18F-FDG injections contained 14.3-19.2 μg/mL for K2.2.2. The iodine-vapor TLC stain method
ISSN:1000-7512
DOI:10.7538/tws.2015.28.03.0129