Cell transcriptomic atlas of the non-human primate Macaca fascicularis

• Published in: Nature (London) Vol. 604; no. 7907; pp. 723 - 731
• Main Authors: Han, Lei, Wei, Xiaoyu, Liu, Chuanyu, Volpe, Giacomo, Zhuang, Zhenkun, Zou, Xuanxuan, Wang, Zhifeng, Pan, Taotao, Yuan, Yue, Zhang, Xiao, Fan, Peng, Guo, Pengcheng, Lai, Yiwei, Lei, Ying, Liu, Xingyuan, Yu, Feng, Shangguan, Shuncheng, Lai, Guangyao, Deng, Qiuting, Liu, Ya, Wu, Liang, Shi, Quan, Yu, Hao, Huang, Yunting, Cheng, Mengnan, Xu, Jiangshan, Liu, Yang, Wang, Mingyue, Wang, Chunqing, Zhang, Yuanhang, Xie, Duo, Yang, Yunzhi, Yu, Yeya, Zheng, Huiwen, Wei, Yanrong, Huang, Fubaoqian, Lei, Junjie, Huang, Waidong, Zhu, Zhiyong, Lu, Haorong, Wang, Bo, Wei, Xiaofeng, Chen, Fengzhen, Yang, Tao, Du, Wensi, Chen, Jing, Xu, Shibo, An, Juan, Ward, Carl, Wang, Zongren, Pei, Zhong, Wong, Chi-Wai, Liu, Xiaolei, Zhang, Huafeng, Liu, Mingyuan, Qin, Baoming, Schambach, Axel, Isern, Joan, Feng, Liqiang, Liu, Yan, Guo, Xiangyu, Liu, Zhen, Sun, Qiang, Maxwell, Patrick H., Barker, Nick, Muñoz-Cánoves, Pura, Gu, Ying, Mulder, Jan, Uhlen, Mathias, Tan, Tao, Liu, Shiping, Yang, Huanming, Wang, Jian, Hou, Yong, Xu, Xun, Esteban, Miguel A., Liu, Longqi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.04.2022; Nature Publishing Group
• Subjects: 13/1; 13/51; 42/62; 59/5; 631/114; 631/443; 631/80; Abdomen; Adipocytes; adult; animal; animal cell; animal experiment; animal tissue; Animals; Body fat; cell; Cell Communication; cell interaction; cell isolation; cell transcriptomic atlas; cellular; controlled study; Datasets; female; Gene expression; genetic analysis; genetic disorder; Genetic disorders; genetics; Humanities and Social Sciences; Infectious diseases; Lymphatic system; Macaca fascicularis; Macaca fascicularis - genetics; male; mouse; multidisciplinary; Musculoskeletal system; nonhuman; phylogenetics; Phylogeny; primate; Primates; Receptors; Receptors, Virus - genetics; RNA sequencing; Science; Science (multidisciplinary); single cell RNA seq; single nucleus RNA sequencing; species comparison; subcellular and molecular biological phenomena and functions; transcriptome; Transcriptome - genetics; Transcriptomics; virus; virus infection; Viruses; Websites; Wnt protein; Wnt signaling; Wnt Signaling Pathway
• ISSN: 0028-0836; 1476-4687; 1476-4687
• DOI: 10.1038/s41586-022-04587-3

Studying tissue composition and function in non-human primates (NHPs) is crucial to understand the nature of our own species. Here we present a large-scale cell transcriptomic atlas that encompasses over 1 million cells from 45 tissues of the adult NHP Macaca fascicularis . This dataset provides a vast annotated resource to study a species phylogenetically close to humans. To demonstrate the utility of the atlas, we have reconstructed the cell–cell interaction networks that drive Wnt signalling across the body, mapped the distribution of receptors and co-receptors for viruses causing human infectious diseases, and intersected our data with human genetic disease orthologues to establish potential clinical associations. Our M .  fascicularis cell atlas constitutes an essential reference for future studies in humans and NHPs. A large-scale single-cell transcriptomic atlas of the non-human primate Macaca fascicularis encompasses over 1 million cells from 45 adult tissues.