A novel biocatalyst, Enterobacter aerogenes LU2, for efficient production of succinic acid using whey permeate as a cost-effective carbon source

• Published in: Biotechnology for biofuels Vol. 13; no. 1; p. 96
• Main Authors: Szczerba, Hubert, Komoń-Janczara, Elwira, Dudziak, Karolina, Waśko, Adam, Targoński, Zdzisław
• Format: Journal Article
• Language: English
• Published: London BioMed Central 29.05.2020; BioMed Central Ltd; Nature Publishing Group; BMC
• Subjects: Acids; Aerogenes; batch fermentation; Batch processing; biocatalysts; biofuels; Bioreactors; Biotechnology; By products; Carbon; Carbon sources; Cell growth; Chemical compounds; Chemistry; Chemistry and Materials Science; cost effectiveness; cows; Enterobacter aerogenes; Environmental Engineering/Biotechnology; feedstocks; Fermentation; Genetic analysis; Glucose; Glycerol; Identification; Industrial applications; Inoculum; Lactose; Microbiology; Microorganisms; Phylogenetics; Phylogeny; Plant Breeding/Biotechnology; Productivity; proteomics; Raw materials; Renewable and Green Energy; Rumen; Succinic acid; Sustainable development; Whey; Whey permeate; Yeast; yeast extract; Poland; United Kingdom; Germany; Succinic acid; Lactose; Whey permeate; Fermentation; Enterobacter aerogenes
• ISSN: 1754-6834; 1754-6834; 2731-3654
• DOI: 10.1186/s13068-020-01739-3

Background Succinic acid (SA), a valuable chemical compound with a broad range of industrial uses, has become a subject of global interest in recent years. The bio-based production of SA by highly efficient microbial producers from renewable feedstock is significantly important, regarding the current trend of sustainable development. Results In this study, a novel bacterial strain, LU2, was isolated from cow rumen and recognized as an efficient producer of SA from lactose. Proteomic and genetic identifications as well as phylogenetic analysis were performed, and strain LU2 was classified as an Enterobacter aerogenes species. The optimal conditions for SA production were 100 g/L lactose, 10 g/L yeast extract, and 20% inoculum at pH 7.0 and 34 °C. Under these conditions, approximately 51.35 g/L SA with a yield of 53% was produced when batch fermentation was conducted in a 3-L stirred bioreactor. When lactose was replaced with whey permeate, the highest SA concentration of 57.7 g/L was achieved with a yield and total productivity of 62% and 0.34 g/(L*h), respectively. The highest productivity of 0.67 g/(L*h) was observed from 48 to 72 h of batch fermentation, when E . aerogenes LU2 produced 16.23 g/L SA. Conclusions This study shows that the newly isolated strain E . aerogenes LU2 has great potential as a new biocatalyst for producing SA from whey permeate.