高效液相色谱法分析马铃薯中α-茄碱
1引言α-茄碱是变绿发芽后的马铃薯中含量最高的一种生物碱,具有抗肿瘤、抑菌、强心、镇痛等[1]多种功效。目前对马铃薯中α-茄碱的高效液相色谱检测法(HPLC)报道较少,并存在重现性较差、目标峰难分开等问题。
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          | Published in | 分析化学 Vol. 39; no. 9; pp. 1459 - 1460 | 
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| Main Author | |
| Format | Journal Article | 
| Language | Chinese | 
| Published | 
            湖南农业大学园艺园林学院植物资源工程系,长沙410128%湖南农业大学国家植物功能成分工程技术研究中心,长沙,410128%湖南农业大学园艺园林学院植物资源工程系,长沙,410128
    
        2011
     湖南农业大学国家植物功能成分工程技术研究中心,长沙410128  | 
| Subjects | |
| Online Access | Get full text | 
| ISSN | 0253-3820 | 
| DOI | 10.3724/SP.J.1096.2011.01459 | 
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| Summary: | 1引言α-茄碱是变绿发芽后的马铃薯中含量最高的一种生物碱,具有抗肿瘤、抑菌、强心、镇痛等[1]多种功效。目前对马铃薯中α-茄碱的高效液相色谱检测法(HPLC)报道较少,并存在重现性较差、目标峰难分开等问题。 | 
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| Bibliography: | XIAO Wen-Jun,LI Qin,XIONG Xin-Yao,ZHOU Kai-Jing,ZHANG Sheng,CHENG Ling,REN Guo-Pu (National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University,Changsha 410128) 2(Botanic Resource Engineering Department of Horticure and Landscape College, Hunan Agricultural University,Changsha,410128) 3(Food Science and Engineering College of Central South Forestry University,Changsha 410004) 22-1125/O6 Potato; α-Solanine; High performance liquid chromatography A method was established for the determination of α-solanine in potato by high performance liquid chromatography.The α-solanine was extracted from potato by refluxing extraction.The effects of different mobile phase composition,concentration,concentration ratio,flow speed,column temperature and other factors on α-solanine separation were studied.When the mobile phase was a mixture of acetonitrile and 0.4% phosphoric acid aqueous solution,flow speed was 0.8 mL/min,temperature was 30 ℃,detection wavele  | 
| ISSN: | 0253-3820 | 
| DOI: | 10.3724/SP.J.1096.2011.01459 |