Anti-Inflammatory Effects of Neochlorogenic Acid Extract from Mulberry Leaf (Morus alba L.) Against LPS-Stimulated Inflammatory Response through Mediating the AMPK/Nrf2 Signaling Pathway in A549 Cells

• Published in: Molecules (Basel, Switzerland) Vol. 25; no. 6; p. 1385
• Main Authors: Gao, Xiao-han, Zhang, Sun-dong, Wang, Li-tao, Yu, Liang, Zhao, Xue-lian, Ni, Hai-yan, Wang, Yan-qiu, Wang, Jian-dong, Shan, Chun-hua, Fu, Yu-jie
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.03.2020; MDPI
• Subjects: A549 Cells; AMP-Activated Protein Kinases - metabolism; ampk/nrf2 signaling pathway; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; anti-inflammatory effect; Chlorogenic Acid - analogs & derivatives; Chlorogenic Acid - chemistry; Cytokines; Cytoplasm; Cytotoxicity; Humans; Inflammation; Inflammation - drug therapy; Inflammation - metabolism; Inflammation - pathology; inflammatory response; Kinases; lps; Morus - chemistry; mulberry leaf (morus alba l.); neochlorogenic acid; NF-E2-Related Factor 2 - metabolism; Oxidative stress; Phosphorylation; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Leaves - chemistry; Pneumonia; Proteins; Quinic Acid - analogs & derivatives; Quinic Acid - chemistry; Signal transduction; Signal Transduction - drug effects; Studies; Transcription factors; Tumor necrosis factor-TNF; inflammatory response; neochlorogenic acid; anti-inflammatory effect; mulberry leaf (Morus alba L.); AMPK/Nrf2 signaling pathway; LPS
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25061385

Neochlorogenic acid (nCGA) is a phenolic compound isolated from mulberry leaf (Morus alba L.), which possesses multiple pharmacological activities containing antioxidant and anti-inflammatory effects. However, the role of nCGA in the treatment of acute pneumonia and the underlying molecular mechanism are still unclear. Hence, the aim of study is to investigate the anti-inflammatory properties of nCGA on LPS-stimulated inflammation in A549 cells. In the present study, results reported that nCGA without cytotoxicity significantly reduced the production of TNF-α, IL-6, and NO, and further suppressed the proteins of iNOS, COX2, TNF-α, IL-6 expression. Furthermore, nCGA also inhibited NF-κB activation and blocked MAPKs signaling pathway phosphorylation. In addition, we found nCGA significantly increased the expression of HO-1 via activating the AMPK/Nrf2 signaling pathway to attenuate the inflammatory response, whereas this protective effect of nCGA was reversed by pre-treatment with compound C (C.C, an AMPK inhibitor). Therefore, all these results indicated that nCGA might act as a natural anti-inflammatory agent for the treatment of acute pneumonia.