Transcriptome Analysis of Sunflower Genotypes with Contrasting Oxidative Stress Tolerance Reveals Individual- and Combined- Biotic and Abiotic Stress Tolerance Mechanisms

• Published in: PloS one Vol. 11; no. 6; p. e0157522
• Main Authors: Ramu, Vemanna S., Paramanantham, Anjugam, Ramegowda, Venkategowda, Mohan-Raju, Basavaiah, Udayakumar, Makarla, Senthil-Kumar, Muthappa
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.06.2016; Public Library of Science (PLoS)
• Subjects: Abiotic stress; Biology and Life Sciences; Combined stress; Crops; Damage tolerance; Drought; Droughts; Ecology and Environmental Sciences; Gene expression; Gene Expression Regulation, Plant; Genes; Genomes; Genotype; Genotypes; Heat; Helianthus; Helianthus - genetics; Helianthus - growth & development; Identification; Menadione; Meta-analysis; Metabolism; Oligonucleotide Array Sequence Analysis; Oxidative stress; Oxidative Stress - genetics; Physical Sciences; Physiology; Plant biology; Plant Proteins - biosynthesis; Plant Proteins - genetics; Plant sciences; Plants (botany); Rain; Research and Analysis Methods; Rice; Screening; Seedlings; Seeds; Studies; Tobacco; Transcription; Transcription factors; Transcriptome - genetics; United States > US; India
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0157522

In nature plants are often simultaneously challenged by different biotic and abiotic stresses. Although the mechanisms underlying plant responses against single stress have been studied considerably, plant tolerance mechanisms under combined stress is not understood. Also, the mechanism used to combat independently and sequentially occurring many number of biotic and abiotic stresses has also not systematically studied. From this context, in this study, we attempted to explore the shared response of sunflower plants to many independent stresses by using meta-analysis of publically available transcriptome data and transcript profiling by quantitative PCR. Further, we have also analyzed the possible role of the genes so identified in contributing to combined stress tolerance. Meta-analysis of transcriptomic data from many abiotic and biotic stresses indicated the common representation of oxidative stress responsive genes. Further, menadione-mediated oxidative stress in sunflower seedlings showed similar pattern of changes in the oxidative stress related genes. Based on this a large scale screening of 55 sunflower genotypes was performed under menadione stress and those contrasting in oxidative stress tolerance were identified. Further to confirm the role of genes identified in individual and combined stress tolerance the contrasting genotypes were individually and simultaneously challenged with few abiotic and biotic stresses. The tolerant hybrid showed reduced levels of stress damage both under combined stress and few independent stresses. Transcript profiling of the genes identified from meta-analysis in the tolerant hybrid also indicated that the selected genes were up-regulated under individual and combined stresses. Our results indicate that menadione-based screening can identify genotypes not only tolerant to multiple number of individual biotic and abiotic stresses, but also the combined stresses.