Comparative genomic analysis and phenazine production of Pseudomonas chlororaphis, a plant growth-promoting rhizobacterium

• Published in: Genomics data Vol. 4; no. C; pp. 33 - 42
• Main Authors: Chen, Yawen, Shen, Xuemei, Peng, Huasong, Hu, Hongbo, Wang, Wei, Zhang, Xuehong
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2015; Elsevier
• Subjects: Anti-bacterial activity; antibiotics; biosynthesis; Comparative genomics; genes; genomics; insecticidal proteins; metabolites; Phenazines; phylogeny; plant growth-promoting rhizobacteria; Pseudomonas; Pseudomonas chlororaphis; Regular; Rhizosphere colonization; virulence; Tad pili; Comparative genomics; MLSA; HPR; Pseudomonas; Anti-bacterial activity; Prn; Rhizosphere colonization; mGS; PGPR; ACC; GI; COGs; 2-OH-PHZ; IAA; AAI; PCA; Fit; Acr; Mcf; PAA; PQQ; HCN; PCN; MCP; Phenazines; Pvd; Prn, pyrrolnitrin; MCP, methyl-accepting chemotaxis protein; Tad pili, type IVb tight adherence pili; PAA, phenylacetic acid; PCA, phenazine-1-carboxylic acid; 2-OH-PHZ, 2-hydroxyphenazine; IAA, indole-3-acetic acid; PGPR, plant growth-promoting rhizobacteria; HPR, 2-hexyl-5-propyl-alkylresorcinol; Acr, achromobactin; HCN, hydrogen cyanide; COGs, Clusters of Orthologous Groups; AAI, amino acid identity; Pvd, pyoverdin; ACC, 1-aminocyclopropane-1-carboxylate; MLSA, multilocus sequence analysis; mGS, mGenomeSubtractor; Fit, P. fluorescens insecticidal toxin; GI, genomic island; Mcf, makes caterpillars floppy; PCN, phenazine-1-carboxamide; PQQ, pyrroloquinoline quinine
• ISSN: 2213-5960; 2213-5960
• DOI: 10.1016/j.gdata.2015.01.006

Pseudomonas chlororaphis HT66, a plant growth-promoting rhizobacterium that produces phenazine-1-carboxamide with high yield, was compared with three genomic sequenced P. chlororaphis strains, GP72, 30–84 and O6. The genome sizes of four strains vary from 6.66 to 7.30Mb. Comparisons of predicted coding sequences indicated 4833 conserved genes in 5869–6455 protein-encoding genes. Phylogenetic analysis showed that the four strains are closely related to each other. Its competitive colonization indicates that P. chlororaphis can adapt well to its environment. No virulence or virulence-related factor was found in P. chlororaphis. All of the four strains could synthesize antimicrobial metabolites including different phenazines and insecticidal protein FitD. Some genes related to the regulation of phenazine biosynthesis were detected among the four strains. It was shown that P. chlororaphis is a safe PGPR in agricultural application and could also be used to produce some phenazine antibiotics with high-yield. •The comparative genomic analysis showed that P. chlororaphis strains have 80% conserved genes.•Its competitive colonization indicates that P. chlororaphis can adapt well to its environment.•P. chlororaphis can synthesize different phenazine compounds and insecticidal proteins.•The plant growth-promoting activities and lack of virulence factor make P. chlororaphis suitable for applications.