Antiosteoporotic Activity of Anthraquinones from Morinda officinalis on Osteoblasts and Osteoclasts

• Published in: Molecules (Basel, Switzerland) Vol. 14; no. 1; pp. 573 - 583
• Main Authors: Wu, Yan-Bin, Zheng, Cheng-Jian, Qin, Lu-Ping, Sun, Lian-Na, Han, Ting, Jiao, Lei, Zhang, Qiao-Yan, Wu, Jin-Zhong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23.01.2009; Molecular Diversity Preservation International
• Subjects: Alkaline Phosphatase - metabolism; Animals; Anthraquinones; Anthraquinones - chemistry; Anthraquinones - pharmacology; Anthraquinones - therapeutic use; Bone Density Conservation Agents - chemistry; Bone Density Conservation Agents - pharmacology; Bone Density Conservation Agents - therapeutic use; Bone Resorption - drug therapy; Cell Proliferation - drug effects; Cells, Cultured; Fractionation; Molecular Structure; Morinda - chemistry; Morinda officinalis; Osteoblast; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoclast; Osteoclasts - cytology; Osteoclasts - drug effects; Osteoporosis; Pharmacy; Phosphatase; Rats; Rats, Wistar; Traditional Chinese medicine; China
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules14010573

Bioactivity-guided fractionation led to the successful isolation of antiosteoporotic components, i.e. physicion (1), rubiadin-1-methyl ether (2), 2-hydroxy-1-methoxy- anthraquinone (3), 1,2-dihydroxy-3-methylanthraquinone (4), 1,3,8-trihydroxy-2-methoxy- anthraquinone (5), 2-hydroxymethyl-3-hydroxyanthraquinone (6), 2-methoxyanthraquinone (7) and scopoletin (8) from an ethanolic extract of the roots of Morinda officinalis. Compounds 4-8 are isolated for the first time from M. officinalis. Among them, compounds 2 and 3 promoted osteoblast proliferation, while compounds 4, 5 increased osteoblast ALP activity. All of the isolated compounds inhibited osteoclast TRAP activity and bone resorption, and the inhibitory effects on osteoclastic bone resorption of compounds 1 and 5 were stronger than that of other compounds. Taken together, antiosteoporotic activity of M. officinalis and its anthraquinones suggest therapeutic potential against osteoporosis.