肝移植后患者外周血Tfh细胞亚群的变化特征及意义

目的探讨肝移植术前和术后患者外周血滤泡辅助性T细胞（Tfh）亚群的表达变化及其与预后的相关性。方法对11例肝移植术后肝功能稳定的患者进行随访研究,采用流式细胞术检测移植前和移植后4周外周血中Tfh细胞亚群频率的变化。结果与移植术前比较,移植术后4周患者外周血Tfh1（CD4^＋CXCR5^＋CXCR3^＋CCR6^-）细胞频率较移植前有下降趋势,但差异无统计学意义（P〉0.05）,Tfh2（CD4^＋CXCR5^＋CXCR3^-CCR6^-）细胞频率较移植前明显升高（P〈0.05）,Tfh17（CD4^＋CXCR5^＋CXCR3^–CCR6^＋）细胞频率较移植前有上升趋势,但差异无统计学意义（...

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Published inJie fang jun yi xue za zhi Vol. 41; no. 10; pp. 833 - 836
Main Author 张可 施明 孙艳玲 王立峰 王洪波 刘鸿凌 刘振文 金磊 王福生
Format Journal Article
LanguageChinese
Published Beijing People's Military Medical Press 2016
Subjects
Liver
Transplants & implants
滤泡辅助性T细胞
移植免疫学
肝移植
Online AccessGet full text
ISSN0577-7402
DOI10.11855/j.issn.0577-7402.2016.10.08

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Summary:目的探讨肝移植术前和术后患者外周血滤泡辅助性T细胞（Tfh）亚群的表达变化及其与预后的相关性。方法对11例肝移植术后肝功能稳定的患者进行随访研究,采用流式细胞术检测移植前和移植后4周外周血中Tfh细胞亚群频率的变化。结果与移植术前比较,移植术后4周患者外周血Tfh1（CD4^＋CXCR5^＋CXCR3^＋CCR6^-）细胞频率较移植前有下降趋势,但差异无统计学意义（P〉0.05）,Tfh2（CD4^＋CXCR5^＋CXCR3^-CCR6^-）细胞频率较移植前明显升高（P〈0.05）,Tfh17（CD4^＋CXCR5^＋CXCR3^–CCR6^＋）细胞频率较移植前有上升趋势,但差异无统计学意义（P〉0.05）。结论 Tfh2和Tfh17细胞可能与肝移植后患者肝功能的恢复有关。
Bibliography:liver transplantation; follicular helper T cells; transplant immunology
Objective To investigate the expression of follicular helper T（Tfh） cell subsets in peripheral blood of patients with liver transplantation（LT） and relevance to the prognosis. Methods Eleven liver transplant patients with stable liver function were enrolled in this study. The frequencies of Tfh subsets were examined by flow cytometry. The frequencies and the level of alanine aminotransferase（ALT）, aspartate transaminase（AST） and total bilirubin（TBIL） were monitored dynamically within one month after LT. Results The frequency of CD4^＋CXCR5^＋CXCR3^-CCR6^- Tfh2 subset in peripheral blood increased significantly after LT as compared with those before transplantation（P〈0.05）. In addition, the frequency of CD4^＋CXCR5^＋CXCR3^–CCR6^＋ Tfh17 subset in peripheral blood showed a trend of increase. The frequency of CD4^＋CXCR5^＋CXCR3^＋CCR6^- Tfh1 subset, however, showed a downtrend, but no statistical difference was found. Conclusion The subsets of Tfh2
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ISSN:0577-7402
DOI:10.11855/j.issn.0577-7402.2016.10.08