Detection of Mycobacterial DNA in Human Bone Marrow

• Published in: Microorganisms (Basel) Vol. 11; no. 7; p. 1788
• Main Authors: González-Escalada, Alba, Rebollo, María José, Barrios Payan, Jorge, Hernández-Pando, Rogelio, García, María Jesús
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11.07.2023; MDPI
• Subjects: Adipocytes; Biopsy; Body fat; Bone marrow; carrier state; Deoxyribonucleic acid; Disease; DNA; Genetic aspects; granuloma; Granulomas; Hematology; Hematopoiesis; Hemopoiesis; HIV; Homeostasis; human bone marrow; Human immunodeficiency virus; humans; Hybridization; Identification and classification; Immune system; Infections; Latent infection; Lipids; Macrophages; mycobacterial diseases; mycobacterial DNA; Mycobacterium avium; Mycobacterium tuberculosis; Paraffin; Pathogens; Physiological aspects; Polymerase chain reaction; Reticuloendothelial system; Testing; Tuberculosis; tuberculosis infection; Spain; Madrid Spain; mycobacterial DNA; tuberculosis infection; adipocytes; human bone marrow
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11071788

Bone marrow is a cell-rich tissue of the reticuloendothelial system essential in the homeostasis and accurate functioning of hematopoiesis and of the immune system; moreover, it is also rich in lipids because it contains marrow adipocytes. This work aimed to evaluate the detection of mycobacterial DNA in human bone marrow as a tool to understand the complex pathology caused by the main pathogen Mycobacterium tuberculosis (Mtb). Formalin-fixed paraffin-embedded human bone marrow samples were studied using both conventional PCR + hybridization and in situ PCR to figure out the cell distribution of the targeted DNA. Samples were retrospectively collected from HIV+ patients with microbiologically proved mycobacterial infection and from subjects without evidence of infection. Mycobacterium avium (Mav) as well as Mtb DNA was detected in both settings, including tissues with and without granulomas. We detected DNA from both mycobacterial species, using in situ PCR, inside bone marrow macrophages. Other cell types, including adipocytes, showed positive signals only for Mtb DNA. This result suggested, for the first time, that marrow adipocytes could constitute an ideal reservoir for the persistence of Mtb, allowing the bacilli to establish long-lasting latent infection within a suitable lipid environment. This fact might differentiate pathogenic behavior of non-specialized pathogens such as Mav from that of specialized pathogens such as Mtb.