High-Level Resistance of Nosema ceranae, a Parasite of the Honeybee, to Temperature and Desiccation

• Published in: Applied and Environmental Microbiology Vol. 75; no. 21; pp. 6886 - 6889
• Main Authors: Fenoy, S, Rueda, C, Higes, M, Martín-Hernández, R, del Aguila, C
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.11.2009; American Society for Microbiology (ASM)
• Subjects: 4',6-Diamidino-2-phenylindole; Animals; Apis mellifera; Bees; Bees - microbiology; Biological and medical sciences; Desiccation; Freezing; Fundamental and applied biological sciences. Psychology; heat tolerance; honey bees; Hot Temperature; Indoles - metabolism; Invertebrate Microbiology; Microbial Viability; Microbiology; Nosema - isolation & purification; Nosema - physiology; Nosema - radiation effects; Nosema ceranae; Organic Chemicals - metabolism; Parasites; spores; Spores, Fungal - physiology; Spores, Fungal - radiation effects; Staining and Labeling - methods; Stress, Physiological; Temperature; viability; Fungi; Infection; Resistance; Temperature; Desiccation; Parasite; Nosema; Parasitosis; Microsporidia
• ISSN: 0099-2240; 1098-5336; 1098-5336; 1098-6596
• DOI: 10.1128/AEM.01025-09

Resistance of Nosema ceranae to different exposure conditions has been evaluated by using Sytox green and DAPI (4',6-diamidino-2-phenylindole) to test spore viability. High thermotolerance at 60 and 35°C and resistance to desiccation were observed. However, a significant decrease in viability after freezing and a rapid degeneration of spores maintained at 4°C were also detected.