Using barcoded Zika virus to assess virus population structure in vitro and in Aedes aegypti mosquitoes

Arboviruses such as Zika virus (ZIKV, Flaviviridae; Flavivirus) must replicate in both mammalian and insect hosts possessing strong immune defenses. Accordingly, transmission between and replication within hosts involves genetic bottlenecks, during which viral population size and genetic diversity m...

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Published inVirology (New York, N.Y.) Vol. 521; pp. 138 - 148
Main Authors Weger-Lucarelli, James, Garcia, Selene M., Rückert, Claudia, Byas, Alex, O’Connor, Shelby L., Aliota, Matthew T., Friedrich, Thomas C., O’Connor, David H., Ebel, Gregory D.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.08.2018
Subjects
Aedes - virology
Aedes aegypti
Animals
Arbovirus
arboviruses
Cell Line
Flavivirus
Genetic Variation
genome
Haplorhini - virology
high-throughput nucleotide sequencing
hosts
immune response
Infectious Disease
insects
mammals
Mosquito-borne virus
population size
population structure
Virus evolution
Zika virus
Zika Virus - classification
Zika Virus - genetics
Zika Virus - growth & development
Mosquito-borne virus
Virus evolution
Zika virus
Arbovirus
Flavivirus
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ISSN0042-6822
1096-0341
1096-0341
DOI10.1016/j.virol.2018.06.004

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Summary:Arboviruses such as Zika virus (ZIKV, Flaviviridae; Flavivirus) must replicate in both mammalian and insect hosts possessing strong immune defenses. Accordingly, transmission between and replication within hosts involves genetic bottlenecks, during which viral population size and genetic diversity may be significantly reduced. To help quantify these bottlenecks and their effects, we constructed 4 “barcoded” ZIKV populations that theoretically contain thousands of barcodes each. After identifying the most diverse barcoded virus, we passaged this virus 3 times in 2 mammalian and mosquito cell lines and characterized the population using deep sequencing of the barcoded region of the genome. C6/36 maintain higher barcode diversity, even after 3 passages, than Vero. Additionally, field-caught mosquitoes exposed to the virus to assess bottlenecks in a natural host. A progressive reduction in barcode diversity occurred throughout systemic infection of these mosquitoes. Differences in bottlenecks during systemic spread were observed between different populations of Aedes aegypti.
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Current address: Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia.
ISSN:0042-6822
1096-0341
1096-0341
DOI:10.1016/j.virol.2018.06.004