Investigation of "Signal" Constituents for the Evaluation of Animal Crude Drugs. I. Free Amino Acids and Total Amino Acids

• Published in: Chem. Pharm. Bull Vol. 42; no. 8; pp. 1636 - 1641
• Main Authors: YAMASAKI, Kazuya, KOKUSENYA, Yoshio, HASHIMOTO, Akio, MIYAMOTO, Takaaki, SATO, Tadashi
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1994; 公益社団法人日本薬学会; Maruzen; Japan Science and Technology Agency
• Subjects: 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazole; amino acid; Amino Acids - analysis; Analysis; animal crude drug; Animals; Biological and medical sciences; Chromatography, High Pressure Liquid; Drug Evaluation; General pharmacology; Medical sciences; Medicine, Chinese Traditional; orthophthalaldehyde; Pharmaceutical Preparations - chemistry; Pharmacology. Drug treatments; precolumn derivatization HPLC; Prospective Studies; Spectrometry, Fluorescence; Drug; α-Aminoacid; HPLC chromatography; Animal origin; Chemical composition; Derivatization; Postcolumn; Quantitative analysis
• ISSN: 0009-2363; 1347-5223; 1347-5223
• DOI: 10.1248/cpb.42.1636

The contents of free amino acids (FAA) and total amino acids (TAA; including peptide and protein forms) in 10 animal drugs were determined using precolumn-derivatization high performance liquid chromatography (HPLC). The results showed that the FAA and TAA content was characteristic for a particular animal crude drug. For example, Bezoar Bovis contained much more taurine relative to the FAA and TAA content compared with other crude drugs, and kokurozin contained much more TAA and much less secondary amino acids relative to TAA compared with koukuzin. FAA and TAA were proved to be "signal" constituents for the evaluation of animal crude drugs.