Efficient apoptosis requires feedback amplification of upstream apoptotic signals by effector caspase-3 or -7

NRC publication: No

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Bibliographic Details
Published inScience advances Vol. 5; no. 7; p. eaau9433
Main Authors McComb, Scott, Chan, Pik Ki, Guinot, Anna, Hartmannsdottir, Holmfridur, Jenni, Silvia, Dobay, Maria Pamela, Bourquin, Jean-Pierre, Bornhauser, Beat C
Format Journal Article
LanguageEnglish
Published United States American Association for the Advancement of Science 01.07.2019
Subjects
Apoptosis - genetics
Caspase 3 - genetics
Caspase 7 - genetics
Caspase 8 - genetics
Caspase 9 - genetics
Cell Biology
Cell Polarity - genetics
Cytochromes c - genetics
Feedback, Physiological
Gene Knockout Techniques
Humans
Mitochondria - genetics
SciAdv r-articles
Signal Transduction - genetics
Online AccessGet full text
ISSN2375-2548
2375-2548
DOI10.1126/sciadv.aau9433

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Abstract NRC publication: No
Apoptosis is a complex multi-step process driven by caspase-dependent proteolytic cleavage cascades. Dysregulation of apoptosis promotes tumorigenesis and limits the efficacy of chemotherapy. To assess the complex interactions among caspases during apoptosis, we disrupted caspase-8, -9, -3, -7, or -6 and combinations thereof, using CRISPR-based genome editing in living human leukemia cells. While loss of apical initiator caspase-8 or -9 partially blocked extrinsic or intrinsic apoptosis, respectively, only combined loss of caspase-3 and -7 fully inhibited both apoptotic pathways, with no discernible effect of caspase-6 deficiency alone or in combination. Caspase-3/7 double knockout cells exhibited almost complete inhibition of caspase-8 or -9 activation. Furthermore, deletion of caspase-3 and -7 decreased mitochondrial depolarization and cytochrome c release upon apoptosis activation. Thus, activation of effector caspase-3 or -7 sets off explosive feedback amplification of upstream apoptotic events, which is a key feature of apoptotic signaling essential for efficient apoptotic cell death.
AbstractList Caspase-3 and -7 are redundantly required to amplify the extrinsic and intrinsic apoptotic cascade in human leukemia. Apoptosis is a complex multi-step process driven by caspase-dependent proteolytic cleavage cascades. Dysregulation of apoptosis promotes tumorigenesis and limits the efficacy of chemotherapy. To assess the complex interactions among caspases during apoptosis, we disrupted caspase-8, -9, -3, -7, or -6 and combinations thereof, using CRISPR-based genome editing in living human leukemia cells. While loss of apical initiator caspase-8 or -9 partially blocked extrinsic or intrinsic apoptosis, respectively, only combined loss of caspase-3 and -7 fully inhibited both apoptotic pathways, with no discernible effect of caspase-6 deficiency alone or in combination. Caspase-3/7 double knockout cells exhibited almost complete inhibition of caspase-8 or -9 activation. Furthermore, deletion of caspase-3 and -7 decreased mitochondrial depolarization and cytochrome c release upon apoptosis activation. Thus, activation of effector caspase-3 or -7 sets off explosive feedback amplification of upstream apoptotic events, which is a key feature of apoptotic signaling essential for efficient apoptotic cell death.
NRC publication: No
Apoptosis is a complex multi-step process driven by caspase-dependent proteolytic cleavage cascades. Dysregulation of apoptosis promotes tumorigenesis and limits the efficacy of chemotherapy. To assess the complex interactions among caspases during apoptosis, we disrupted caspase-8, -9, -3, -7, or -6 and combinations thereof, using CRISPR-based genome editing in living human leukemia cells. While loss of apical initiator caspase-8 or -9 partially blocked extrinsic or intrinsic apoptosis, respectively, only combined loss of caspase-3 and -7 fully inhibited both apoptotic pathways, with no discernible effect of caspase-6 deficiency alone or in combination. Caspase-3/7 double knockout cells exhibited almost complete inhibition of caspase-8 or -9 activation. Furthermore, deletion of caspase-3 and -7 decreased mitochondrial depolarization and cytochrome c release upon apoptosis activation. Thus, activation of effector caspase-3 or -7 sets off explosive feedback amplification of upstream apoptotic events, which is a key feature of apoptotic signaling essential for efficient apoptotic cell death.
Apoptosis is a complex multi-step process driven by caspase-dependent proteolytic cleavage cascades. Dysregulation of apoptosis promotes tumorigenesis and limits the efficacy of chemotherapy. To assess the complex interactions among caspases during apoptosis, we disrupted caspase-8, -9, -3, -7, or -6 and combinations thereof, using CRISPR-based genome editing in living human leukemia cells. While loss of apical initiator caspase-8 or -9 partially blocked extrinsic or intrinsic apoptosis, respectively, only combined loss of caspase-3 and -7 fully inhibited both apoptotic pathways, with no discernible effect of caspase-6 deficiency alone or in combination. Caspase-3/7 double knockout cells exhibited almost complete inhibition of caspase-8 or -9 activation. Furthermore, deletion of caspase-3 and -7 decreased mitochondrial depolarization and cytochrome c release upon apoptosis activation. Thus, activation of effector caspase-3 or -7 sets off explosive feedback amplification of upstream apoptotic events, which is a key feature of apoptotic signaling essential for efficient apoptotic cell death.Apoptosis is a complex multi-step process driven by caspase-dependent proteolytic cleavage cascades. Dysregulation of apoptosis promotes tumorigenesis and limits the efficacy of chemotherapy. To assess the complex interactions among caspases during apoptosis, we disrupted caspase-8, -9, -3, -7, or -6 and combinations thereof, using CRISPR-based genome editing in living human leukemia cells. While loss of apical initiator caspase-8 or -9 partially blocked extrinsic or intrinsic apoptosis, respectively, only combined loss of caspase-3 and -7 fully inhibited both apoptotic pathways, with no discernible effect of caspase-6 deficiency alone or in combination. Caspase-3/7 double knockout cells exhibited almost complete inhibition of caspase-8 or -9 activation. Furthermore, deletion of caspase-3 and -7 decreased mitochondrial depolarization and cytochrome c release upon apoptosis activation. Thus, activation of effector caspase-3 or -7 sets off explosive feedback amplification of upstream apoptotic events, which is a key feature of apoptotic signaling essential for efficient apoptotic cell death.
Author Guinot, Anna
Hartmannsdottir, Holmfridur
Bourquin, Jean-Pierre
McComb, Scott
Chan, Pik Ki
Jenni, Silvia
Dobay, Maria Pamela
Bornhauser, Beat C
Author_xml – sequence: 1
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  fullname: Dobay, Maria Pamela
– sequence: 7
  fullname: Bourquin, Jean-Pierre
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  fullname: Bornhauser, Beat C
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Cites_doi 10.1016/j.bbamcr.2008.12.004
10.1182/blood-2014-06-582551
10.1111/imr.12541
10.1016/j.ccell.2016.11.011
10.1038/34112
10.1091/mbc.e06-04-0263
10.1038/sj.cdd.4401160
10.1016/S0092-8674(00)00008-8
10.1073/pnas.0707715105
10.1016/j.bbamcr.2011.01.026
10.1016/j.cell.2014.11.036
10.1128/MMBR.64.4.821-846.2000
10.1038/cdd.2011.30
10.1074/jbc.M404893200
10.1016/S0092-8674(00)00009-X
10.1083/jcb.144.2.281
10.1074/jbc.M702969200
10.1016/S0092-8674(00)81590-1
10.1084/jem.188.5.919
10.1038/nature01101
10.1016/j.semcdb.2018.01.009
10.1038/sj.onc.1208793
10.1101/cshperspect.a008656
10.1016/0092-8674(93)90485-9
10.1042/BJ20070288
10.1182/blood-2008-09-178038
10.1182/blood-2007-09-114314
10.1098/rsob.130031
10.1038/sj.cdd.4400589
10.1126/science.1115035
10.1016/j.cell.2014.11.037
10.1016/j.cell.2011.02.013
10.1038/nrm2952
10.1007/s10495-006-0084-y
10.1074/jbc.M204783200
10.1016/j.semcdb.2018.01.002
10.1007/s10495-011-0691-0
10.4172/2155-9899.1000126
10.1016/j.stem.2008.03.012
10.21769/BioProtoc.2222
10.1038/sj.cdd.4401263
10.1126/scitranslmed.aad2986
10.1038/sj.cdd.4401963
10.1074/jbc.274.30.21155
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References e_1_3_2_26_2
e_1_3_2_27_2
e_1_3_2_28_2
e_1_3_2_29_2
e_1_3_2_41_2
e_1_3_2_40_2
e_1_3_2_20_2
e_1_3_2_43_2
e_1_3_2_21_2
e_1_3_2_42_2
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References_xml – ident: e_1_3_2_33_2
  doi: 10.1016/j.bbamcr.2008.12.004
– ident: e_1_3_2_39_2
  doi: 10.1182/blood-2014-06-582551
– ident: e_1_3_2_3_2
  doi: 10.1111/imr.12541
– ident: e_1_3_2_44_2
  doi: 10.1016/j.ccell.2016.11.011
– ident: e_1_3_2_11_2
  doi: 10.1038/34112
– ident: e_1_3_2_23_2
  doi: 10.1091/mbc.e06-04-0263
– ident: e_1_3_2_10_2
  doi: 10.1038/sj.cdd.4401160
– ident: e_1_3_2_32_2
  doi: 10.1016/S0092-8674(00)00008-8
– ident: e_1_3_2_19_2
  doi: 10.1073/pnas.0707715105
– ident: e_1_3_2_28_2
  doi: 10.1016/j.bbamcr.2011.01.026
– ident: e_1_3_2_40_2
  doi: 10.1016/j.cell.2014.11.036
– ident: e_1_3_2_18_2
  doi: 10.1128/MMBR.64.4.821-846.2000
– ident: e_1_3_2_21_2
  doi: 10.1038/cdd.2011.30
– ident: e_1_3_2_12_2
  doi: 10.1074/jbc.M404893200
– ident: e_1_3_2_31_2
  doi: 10.1016/S0092-8674(00)00009-X
– ident: e_1_3_2_9_2
  doi: 10.1083/jcb.144.2.281
– ident: e_1_3_2_25_2
  doi: 10.1074/jbc.M702969200
– ident: e_1_3_2_30_2
  doi: 10.1016/S0092-8674(00)81590-1
– ident: e_1_3_2_27_2
  doi: 10.1084/jem.188.5.919
– ident: e_1_3_2_37_2
  doi: 10.1038/nature01101
– ident: e_1_3_2_4_2
  doi: 10.1016/j.semcdb.2018.01.009
– ident: e_1_3_2_43_2
  doi: 10.1038/sj.onc.1208793
– ident: e_1_3_2_6_2
  doi: 10.1101/cshperspect.a008656
– ident: e_1_3_2_5_2
  doi: 10.1016/0092-8674(93)90485-9
– ident: e_1_3_2_35_2
  doi: 10.1042/BJ20070288
– ident: e_1_3_2_20_2
  doi: 10.1182/blood-2008-09-178038
– ident: e_1_3_2_36_2
  doi: 10.1182/blood-2007-09-114314
– ident: e_1_3_2_29_2
  doi: 10.1098/rsob.130031
– ident: e_1_3_2_15_2
  doi: 10.1038/sj.cdd.4400589
– ident: e_1_3_2_17_2
  doi: 10.1126/science.1115035
– ident: e_1_3_2_41_2
  doi: 10.1016/j.cell.2014.11.037
– ident: e_1_3_2_2_2
  doi: 10.1016/j.cell.2011.02.013
– ident: e_1_3_2_7_2
  doi: 10.1038/nrm2952
– ident: e_1_3_2_14_2
  doi: 10.1007/s10495-006-0084-y
– ident: e_1_3_2_34_2
  doi: 10.1074/jbc.M204783200
– ident: e_1_3_2_8_2
  doi: 10.1016/j.semcdb.2018.01.002
– ident: e_1_3_2_13_2
  doi: 10.1007/s10495-011-0691-0
– ident: e_1_3_2_24_2
  doi: 10.4172/2155-9899.1000126
– ident: e_1_3_2_38_2
  doi: 10.1016/j.stem.2008.03.012
– ident: e_1_3_2_26_2
  doi: 10.21769/BioProtoc.2222
– ident: e_1_3_2_45_2
  doi: 10.1038/sj.cdd.4401263
– ident: e_1_3_2_22_2
  doi: 10.1126/scitranslmed.aad2986
– ident: e_1_3_2_42_2
  doi: 10.1038/sj.cdd.4401963
– ident: e_1_3_2_16_2
  doi: 10.1074/jbc.274.30.21155
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Caspase-3 and -7 are redundantly required to amplify the extrinsic and intrinsic apoptotic cascade in human leukemia. Apoptosis is a complex multi-step process...
Apoptosis is a complex multi-step process driven by caspase-dependent proteolytic cleavage cascades. Dysregulation of apoptosis promotes tumorigenesis and...
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SubjectTerms Apoptosis - genetics
Caspase 3 - genetics
Caspase 7 - genetics
Caspase 8 - genetics
Caspase 9 - genetics
Cell Biology
Cell Polarity - genetics
Cytochromes c - genetics
Feedback, Physiological
Gene Knockout Techniques
Humans
Mitochondria - genetics
SciAdv r-articles
Signal Transduction - genetics
Title Efficient apoptosis requires feedback amplification of upstream apoptotic signals by effector caspase-3 or -7
URI https://nrc-publications.canada.ca/eng/view/object/?id=57baa4c0-af00-4e7e-93ce-2ba886d1d565
https://www.ncbi.nlm.nih.gov/pubmed/31392262
https://www.proquest.com/docview/2270020462
https://pubmed.ncbi.nlm.nih.gov/PMC6669006
Volume 5
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