Determination of Curculigoside in Curculiginis Rhizoma by High Performance Liquid Chromatography

• Published in: Chemical & pharmaceutical bulletin Vol. 42; no. 2; pp. 395 - 397
• Main Authors: YAMASAKI, Kazuya, KOKUSENYA, Yoshio, HASHIMOTO, Akio, MIYAMOTO, Takaaki, SATO, Tadashi, MATSUO, Masaaki
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1994; 公益社団法人日本薬学会; Japan Science and Technology Agency
• Subjects: 2, 6-dimethoxybenzoic acid; Curculiginis Rhizoma; Curculigo orchioides; Curculigoside; HPLC determination; hydrolysis
• ISSN: 0009-2363; 1347-5223; 1347-5223
• DOI: 10.1248/cpb.42.395

An assay method for the determination of curculigoside in Curculiginis Rhizoma by high performacne liquid chromatography (HPLC) was set up. After extraction with ethyl acetate, curculigoside was hydrolyzed in 1 N NaOH, and quantitatively converted to 2, 6-dimethoxybenzoic acid (2, 6-DA). The determination of curculigoside in Curculiginis Rhizoma was performed indirectly by measuring the content of 2, 6-DA by HPLC. The calibration curve for this method exhibited a good linearity with a correlation coefficient of 1.0000 over the concentration range 1.0 to 81.0 μg 2, 6-DA/ml (from 3 to 207 μg/ml as curculigoside). Using this method, 7 lots of Curculiginis Rhizoma produced in China contained about 0.2% curculigoside on average. To estimate the reliability of this method, the curculigoside content of Curculiginis Rhizoma was determined by direct assay applying the HPLC method and using curculigoside as the standard. Both methods agreed very well and this method was found to be satisfactory for estimating the contents of curculigoside in Curculiginis Rhizoma.