Reusable Glucose Sensor Based on Enzyme Immobilized Egg-shell Membrane

• Published in: Analytical Sciences Vol. 32; no. 10; pp. 1077 - 1082
• Main Authors: SINGH, Amit Kumar, JHA, Sandeep Kumar, ROYCHOUDHURY, Appan
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 2016; Springer Nature Singapore; Nature Publishing Group
• Subjects: Analytical Chemistry; Biological properties; Biological samples; Blood tests; Chemistry; Concentration; Egg shells; Egg-shell membrane; Glucose; glucose biosensor; Glucose oxidase; glutaraldehyde immobilization; Membranes; Response time; reusable biosensor; Sensors; Shelf life; Egg-shell membrane; reusable biosensor; glutaraldehyde immobilization; glucose biosensor
• ISSN: 0910-6340; 1348-2246; 1348-2246
• DOI: 10.2116/analsci.32.1077

In this paper, an egg-shell membrane has been used for efficient immobilization and stabilization of glucose oxidase. This membrane was used for developing a simple and reusable method for estimation of glucose in biological samples. The proposed sensor was effectively used in a wide glucose concentration range (1 – 1000 mM) with fast response time of 70 s for higher concentrations and 120 s for lower concentrations. The results of response study for the fabricated sensor show limit of detection of 4.761 mM with high sensitivity over the entire concentration range (1 – 1000 mM). Most interestingly, the membrane used in the fabricated sensor could be repeatedly used for glucose analysis 150 times and it exhibited a shelf-life of more than 6 weeks. The proposed sensor was also demonstrated for estimation of glucose in human blood samples.