T(3;14)(p14.1;q32) involving IGH and FOXP1 is a novel recurrent chromosomal aberration in MALT lymphoma Lymphoma

• Published in: Leukemia Vol. 19; no. 4; pp. 652 - 658
• Main Authors: Streubel, B, Vinatzer, U, Lamprecht, A, Raderer, M, Chott, A
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.04.2005; Nature Publishing; Nature Publishing Group
• Subjects: Abnormalities; Adaptor Proteins, Signal Transducing - genetics; Aged; Aged, 80 and over; B-Cell CLL-Lymphoma 10 Protein; Biological and medical sciences; Cancer Research; Caspases; Chromosome 14; Chromosome 3; Chromosome aberrations; Chromosome translocations; Chromosomes; Chromosomes, Human, Pair 14; Chromosomes, Human, Pair 3; Cloning, Molecular; Critical Care Medicine; Cytogenetics; Exocrine glands; Female; Fluorescence; Fluorescence in situ hybridization; Forkhead Transcription Factors; Foxp1 protein; Genetic abnormalities; Heavy chains; Hematologic and hematopoietic diseases; Hematology; Humans; Hybridization; Immunoglobulin Heavy Chains - genetics; Immunoglobulins; In Situ Hybridization, Fluorescence; Intensive; Internal Medicine; Leukemia; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphoma; Lymphoma, B-Cell, Marginal Zone - genetics; Male; Medical sciences; Medicine; Medicine & Public Health; Middle Aged; Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein; Mucosal-associated lymphoid tissue; Neoplasm Proteins - genetics; Oncology; original-manuscript; Polymerase chain reaction; Repressor Proteins - genetics; RNA-directed DNA polymerase; Salivary gland; Salivary glands; Spleen; Thyroid; Thyroid gland; Translocation; Translocation, Genetic; Trisomy; Tumors; Austria; MALT lymphoma; genetics; BCL10; MALT1; FOXP1; Chromosomal aberration; Heavy peptide chain; Immunoglobulins; Relapse; Hematology; Malignant hemopathy; Non Hodgkin lymphoma; Abnormal chromosome A3; Lymphoproliferative syndrome; Abnormal chromosome D14; Abnormal chromosome; Genetics; Chromosome translocation; Transcription factor FOXP1
• ISSN: 0887-6924; 1476-5551
• DOI: 10.1038/sj.leu.2403644

The three chromosomal translocations t(11;18)(q21;q21), t(14;18)(q32;q21), and t(1;14)(p22;q32) are associated with MALT lymphoma. In a case of MALT lymphoma of the thyroid, we observed t(3;14)(p14.1;q32) by cytogenetic analysis. Fluorescence in situ hybridization studies showed that the immunoglobulin heavy chain locus ( IGH ) was rearranged on chromosome 14. Long-distance inverse polymerase chain reaction identified FOXP1 as the partner gene on chromosome 3. To determine the frequency of the t(3;14)(p14.1;q32), two fluorescence in situ hybridization assays were established to screen 91 MALT lymphomas, all of which were negative for the above-mentioned three translocations, and eight splenic and six nodal marginal zone lymphomas. Overall, nine MALT lymphomas (10%) harbored t(3;14)(p14.1;q32) comprising tumors of the thyroid (three of six), ocular adnexa (four of 20), and skin (two of 20), whereas those of the stomach ( n =20), salivary gland ( n =20), and lung ( n =5) were negative as well as the splenic and nodal marginal zone lymphomas. Most t(3;14)(p14.1;q32)+MALT lymphomas harbored additional genetic abnormalities, such as trisomy 3. Further studies revealed that the three known translocations and t(3;14)(p14.1;q32) are mutually exclusive. Real-time quantitative reverse transcriptase polymerase chain reaction showed upregulation of FOXP1 in cases with t(3;14)(p14.1;q32) or trisomy 3. This study identifies FOXP1 as a new translocation partner of IGH in a site-dependent subset of MALT lymphomas.