Graphene Amination towards Its Grafting by Antibodies for Biosensing Applications

• Published in: Nanomaterials (Basel, Switzerland) Vol. 13; no. 11; p. 1730
• Main Authors: Rabchinskii, Maxim K., Besedina, Nadezhda A., Brzhezinskaya, Maria, Stolyarova, Dina Yu, Ryzhkov, Sergei A., Saveliev, Sviatoslav D., Antonov, Grigorii A., Baidakova, Marina V., Pavlov, Sergei I., Kirilenko, Demid A., Shvidchenko, Aleksandr V., Cherviakova, Polina D., Brunkov, Pavel N.
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.05.2023; MDPI
• Subjects: 2D materials; aminated graphene; Amination; Amines; Antibodies; Biomolecules; Biosensors; Chemical properties; Chemistry; Conjugation; Design and construction; Electron microscopy; Electronic structure; Fourier transforms; Grafting; Graphene; graphene modification; Immobilization; Immunoglobulin G; Immunoglobulin M; Immunoglobulins; Materials; Monoclonal antibodies; Morphology; photoelectron spectroscopy; Photoelectrons; Physiological aspects; Spectroscopy; Viral antibodies; Russia; Germany; photoelectron spectroscopy; 2D materials; grafting; graphene modification; aminated graphene; antibodies; biosensors
• ISSN: 2079-4991; 2079-4991
• DOI: 10.3390/nano13111730

The facile synthesis of biografted 2D derivatives complemented by a nuanced understanding of their properties are keystones for advancements in biosensing technologies. Herein, we thoroughly examine the feasibility of aminated graphene as a platform for the covalent conjugation of monoclonal antibodies towards human IgG immunoglobulins. Applying core-level spectroscopy methods, namely X-ray photoelectron and absorption spectroscopies, we delve into the chemistry and its effect on the electronic structure of the aminated graphene prior to and after the immobilization of monoclonal antibodies. Furthermore, the alterations in the morphology of the graphene layers upon the applied derivatization protocols are assessed by electron microscopy techniques. Chemiresistive biosensors composed of the aerosol-deposited layers of the aminated graphene with the conjugated antibodies are fabricated and tested, demonstrating a selective response towards IgM immunoglobulins with a limit of detection as low as 10 pg/mL. Taken together, these findings advance and outline graphene derivatives’ application in biosensing as well as hint at the features of the alterations of graphene morphology and physics upon its functionalization and further covalent grafting by biomolecules.