Prevalence and Characteristics of Plasmid-Mediated Fosfomycin Resistance Gene fosA3 among Salmonella Enteritidis Isolates from Retail Chickens and Children with Gastroenteritis in China

• Published in: Pathogens (Basel) Vol. 13; no. 9; p. 816
• Main Authors: Liu, Liyuan, Yi, Shanrong, Xu, Xuebin, Zheng, Liya, Liu, Hong, Zhou, Xiujuan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21.09.2024; MDPI
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Antibiotics; Aspergillus nuclease S1; Bacterial genetics; Beta lactamases; Chickens; Chickens - microbiology; Child; Child, Preschool; Children; China; China - epidemiology; Drug Resistance, Bacterial - genetics; E coli; Electrophoresis; Electrophoresis, Gel, Pulsed-Field; Epidemiology; Escherichia coli; fosA3; Fosfomycin; Fosfomycin - pharmacology; Fosfomycin - therapeutic use; Gastroenteritis; Gastroenteritis - drug therapy; Gastroenteritis - epidemiology; Gastroenteritis - microbiology; Genes; Homology; Humans; Methicillin; Microbial Sensitivity Tests; Molecular Epidemiology; Nuclease; Pathogens; plasmid dissemination; Plasmids; Plasmids - genetics; Poultry Diseases - epidemiology; Poultry Diseases - microbiology; Prevalence; Pulsed-field gel electrophoresis; resistance genes; S1-PFGE; Salmonella; Salmonella Enteritidis; Salmonella enteritidis - drug effects; Salmonella enteritidis - genetics; Salmonella enteritidis - isolation & purification; Salmonella Infections - drug therapy; Salmonella Infections - epidemiology; Salmonella Infections - microbiology; Salmonella Infections, Animal - epidemiology; Salmonella Infections, Animal - microbiology; sequence homology; Tetracycline; Tetracyclines; β Lactamase; China; United States > US; molecular epidemiology; plasmid dissemination; S1-PFGE; fosA3; Salmonella Enteritidis
• ISSN: 2076-0817; 2076-0817
• DOI: 10.3390/pathogens13090816

A total of 265 Salmonella Enteritidis isolates collected from retail markets and children’s hospitals in Shanghai were used to investigate the prevalence and molecular epidemiology of plasmid-mediated fosfomycin resistance genes. Nine of the isolates—7 from the 146 (4.79%) retail chicken-related samples and 2 from the 119 (1.68%) samples from clinical children—were fosfomycin-resistant (FosR). The fosA3 gene was detected in all of the nine FosR isolates, which were located on Inc F-type (8/9, 88.9%) and unknown-type (1/9, 11.1%) transferable plasmids. In total, five plasmid types, namely Inc HI2 (1/9, 11.1%), Inc I1 (3/9, 33.3%), Inc X (8/9, 88.9%), Inc FIIs (9/9, 100%), and Inc FIB (9/9, 100%), were detected in these FosR isolates, which possessed five S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) profiles. The extended-spectrum β-lactamase determinant blaCTX-M-14 subtype was identified in one FosR S. Enteritidis isolate, which was located in a transferable unknown-type plasmid co-carrying fosA3 and tetR genes. Sequence homology analysis showed that this plasmid possessed high sequence similarity to previously reported blaCTX-M-14- and fosA3-positive plasmids from E. coli strains, implying that plasmids carrying the fosA3 gene might be disseminated among Enterobacterales. These findings highlight further challenges in the prevention and treatment of Enterobacteriaceae infections caused by plasmids containing fosA3.