Instantaneous isotropic volumetric imaging of fast biological processes

• Published in: NATURE METHODS Vol. 16; no. 6; pp. 497 - 500
• Main Authors: Wagner, Nils, Norlin, Nils, Gierten, Jakob, de Medeiros, Gustavo, Balázs, Bálint, Wittbrodt, Joachim, Hufnagel, Lars, Prevedel, Robert
• Format: Journal Article Publication
• Language: English
• Published: New York Nature Publishing Group US 01.06.2019; Nature Publishing Group
• Subjects: 631/136; 631/1647/245; 631/1647/328; 631/1647/328/2237; 631/57/2266; Algorithms; Analysis; Animals; Animals, Genetically Modified; Artefacts; Bioinformatics; Biological activity; Biological Microscopy; Biological systems; Biological Techniques; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biophysics; Blood flow; Blood Flow Velocity; Brief Communication; Cameras; Heart; Heart - diagnostic imaging; Heart - physiology; Humans; Image acquisition; Image Processing, Computer-Assisted - methods; Image reconstruction; Imaging, Three-Dimensional - methods; Laboratories; Life Sciences; Light; Methods; Microscope and microscopy; Microscopy; Microscopy - methods; Molecular biology; Neurosciences; Objectives; Oryzias; Performance evaluation; Proteomics; Single-Cell Analysis - methods; Spatial discrimination; Spatial resolution; Three dimensional imaging
• ISSN: 1548-7091; 1548-7105; 1548-7105
• DOI: 10.1038/s41592-019-0393-z

To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate medaka heart and blood flow imaging at single-cell resolution and free of motion artefacts at volume rates of up to 200 Hz. Iso-LFM enables rapid, instantaneous volumetric imaging of biological processes with isotropic and improved resolution by simultaneously capturing orthogonal light fields.