ProClaT, a new bioinformatics tool for in silico protein reclassification: case study of DraB, a protein coded from the draTGB operon in Azospirillum brasilense

• Published in: BMC bioinformatics Vol. 17; no. Suppl 18; p. 455
• Main Authors: Rubel, Elisa Terumi, Raittz, Roberto Tadeu, Coimbra, Nilson Antonio da Rocha, Gehlen, Michelly Alves Coutinho, Pedrosa, Fábio de Oliveira
• Format: Journal Article
• Language: English
• Published: London BioMed Central 15.12.2016; BioMed Central Ltd
• Subjects: Algorithms; Azospirillum brasilense - chemistry; Azospirillum brasilense - enzymology; Azospirillum brasilense - genetics; Azospirillum brasilense - metabolism; Bacterial proteins; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bioinformatics; Biomedical and Life Sciences; Computational biology; Computational Biology - instrumentation; Computational Biology - methods; Computational Biology/Bioinformatics; Computer Appl. in Life Sciences; Genes, Bacterial; Genetic aspects; Identification and classification; Life Sciences; Microarrays; Nitrogen Fixation; Nitrogen-fixing microorganisms; Nitrogenase - chemistry; Nitrogenase - genetics; Nitrogenase - metabolism; Operon; Physiological aspects; Brazil; Nitrogenase associated NifO protein; Biological nitrogen fixation; Protein classification; Operon; Nitrogenase; Artificial neural networks; Azospirillum brasilense; Operon draTGB
• ISSN: 1471-2105; 1471-2105
• DOI: 10.1186/s12859-016-1338-5

Background Azopirillum brasilense is a plant-growth promoting nitrogen-fixing bacteria that is used as bio-fertilizer in agriculture. Since nitrogen fixation has a high-energy demand, the reduction of N 2 to NH 4 + by nitrogenase occurs only under limiting conditions of NH 4 + and O 2 . Moreover, the synthesis and activity of nitrogenase is highly regulated to prevent energy waste. In A. brasilense nitrogenase activity is regulated by the products of draG and draT . The product of the draB gene, located downstream in the draTGB operon, may be involved in the regulation of nitrogenase activity by an, as yet, unknown mechanism. Results A deep in silico analysis of the product of draB was undertaken aiming at suggesting its possible function and involvement with DraT and DraG in the regulation of nitrogenase activity in A. brasilense . In this work, we present a new artificial intelligence strategy for protein classification, named ProClaT. The features used by the pattern recognition model were derived from the primary structure of the DraB homologous proteins, calculated by a ProClaT internal algorithm. ProClaT was applied to this case study and the results revealed that the A. brasilense draB gene codes for a protein highly similar to the nitrogenase associated NifO protein of Azotobacter vinelandii . Conclusions This tool allowed the reclassification of DraB/NifO homologous proteins, hypothetical, conserved hypothetical and those annotated as putative arsenate reductase, ArsC, as NifO-like. An analysis of co-occurrence of draB , draT , draG and of other nif genes was performed, suggesting the involvement of draB ( nifO ) in nitrogen fixation, however, without the definition of a specific function.