Cellular mRNA expressions of anti-oxidant factors in the blood of preeclamptic women

• Published in: Prenatal Diagnosis Vol. 29; no. 7; pp. 691 - 696
• Main Authors: Nakamura, Masamitsu, Sekizawa, Akihiko, Purwosunu, Yuditiya, Okazaki, Shiho, Farina, Antonio, Wibowo, Noroyono, Shimizu, Hanako, Okai, Takashi
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.07.2009; Wiley
• Subjects: Adolescent; Adult; anti-oxidant enzymes; Antioxidants; Antioxidants - metabolism; Biological and medical sciences; Blood Cells; Blood Cells - metabolism; Blood Pressure; Catalase; Catalase - genetics; Catalase - metabolism; cellular RNA in maternal blood; Data processing; Delivery. Postpartum. Lactation; Enzymes; Female; fetal cells; Fundamental and applied biological sciences. Psychology; Gene expression; Genetics of eukaryotes. Biological and molecular evolution; Gestation; Gynecology. Andrology. Obstetrics; HELLP Syndrome; HELLP Syndrome - blood; HELLP Syndrome - genetics; HELLP Syndrome - metabolism; HELLP Syndrome - physiopathology; Heme Oxygenase (Decyclizing); Heme Oxygenase (Decyclizing) - genetics; Heme Oxygenase (Decyclizing) - metabolism; Heme Oxygenase-1; Heme Oxygenase-1 - genetics; Heme Oxygenase-1 - metabolism; Humans; Medical sciences; Molecular and cellular biology; nucleic acids and proteins; Oxidative Stress; Oxidative Stress - genetics; Oxidative Stress - physiology; Peripheral blood; Polymerase chain reaction; Pre-Eclampsia; Pre-Eclampsia - blood; Pre-Eclampsia - genetics; Pre-Eclampsia - metabolism; Pre-Eclampsia - physiopathology; Pregnancy; Prenatal diagnosis; Proteinuria; Reverse transcription; RNA; RNA, Messenger; RNA, Messenger - blood; RNA, Messenger - metabolism; Statistics; Superoxide Dismutase; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Young Adult; Pregnancy disorders; RNA; Neonatology; Oxidant; anti-oxidant enzymes; nucleic acids and proteins; Blood; Messenger RNA; Mother; Heme; Female; Genetics; Woman; Nucleic acid; Human; Enzyme; Gynecology; pre-eclampsia; heme oxygenase-1; Pregnancy toxemia; Protein; Oxygenase; Fetal cell; Preeclampsia; cellular RNA in maternal blood; fetal cells; Oxidoreductases
• ISSN: 0197-3851; 1097-0223; 1097-0223
• DOI: 10.1002/pd.2278

Objective To assess the alterations of mRNA expressions associated with oxidative stress in the cellular component of blood from pregnant women with pre‐eclampsia. Methods Peripheral blood samples were obtained from pregnant women with and without pre‐eclampsia. Cellular RNA was subjected to a reverse transcription polymerase chain reaction (PCR) assay in order to examine the mRNA distribution among women with pre‐eclampsia (n = 24) and control subjects (n = 24) during 35–41 weeks of gestation. The data were analyzed by non‐parametric statistics. Results Significant differences between the pre‐eclampsia subjects and the controls were observed in the gene expressions associated with oxidative stress. Lower values in the pre‐eclampsia group were found for heme oxygenase (HO)‐1, HO‐2, catalase and superoxide dismutase (SOD). The HO‐1, HO‐2 and the catalase levels significantly correlated with proteinuria, and the HO‐2 level with systolic blood pressure. Conclusion Significantly lower concentrations of HO‐1, HO‐2, SOD and catalase are found in the cellular component of blood from pre‐eclamptic patients. The values correlate with the severity of pre‐eclampsia. These findings indicate that enhanced oxidative stress and a decrease in the number of anti‐oxidant enzymes may be associated with pre‐eclampsia. Copyright © 2009 John Wiley & Sons, Ltd.