Multifunctional two-component in-situ hydrogel for esophageal submucosal dissection for mucosa uplift, postoperative wound closure and rapid healing

• Published in: Bioactive materials Vol. 27; pp. 461 - 473
• Main Authors: Lei, Xiong-Xin, Hu, Juan-Juan, Zou, Chen-Yu, Jiang, Yan-Lin, Zhao, Long-Mei, Zhang, Xiu-Zhen, Li, Ya-Xing, Peng, An-Ni, Song, Yu-Ting, Huang, Li-Ping, Li-Ling, Jesse, Xie, Hui-Qi
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.09.2023; KeAi Publishing; KeAi Communications Co., Ltd
• Subjects: Endoscopic submucosal dissection; Hydrogel; Mucosa uplift; Tissue sealant; Wound healing; GI; Wound healing; CMCS; AM; SA; CS; Endoscopic submucosal dissection; SFC; ESD; Mucosa uplift; Hydrogel; Tissue sealant; ADA
• ISSN: 2452-199X; 2452-199X
• DOI: 10.1016/j.bioactmat.2023.04.015

Endoscopic submucosal dissection (ESD) for gastrointestinal tumors and premalignant lesions needs submucosal fluid cushion (SFC) for mucosal uplift before dissection, and wound care including wound closure and rapid healing postoperatively. Current SFC materials as well as materials and/or methods for post-ESD wound care have single treatment effect and hold corresponding drawbacks, such as easy dispersion, short duration, weak hemostasis and insufficient repair function. Thus, designing materials that can serve as both SFC materials and wound care is highly desired, and remains a challenge. Herein, we report a two-component in-situ hydrogel prepared from maleimide-based oxidized sodium alginate and sulfhydryl carboxymethyl-chitosan, which gelated mainly based on "click" chemistry and Schiff base reaction. The hydrogels showed short gelation time, outstanding tissue adhesion, favorable hemostatic properties, and good biocompatibility. A rat subcutaneous ultrasound model confirmed the ability of suitable mucosal uplift height and durable maintenance time of AM solution. The in vivo/in vitro rabbit liver hemorrhage model demonstrated the effects of hydrogel in rapid hemostasis and prevention of delayed bleeding. The canine esophageal ESD model corroborated that the in-situ hydrogel provided good mucosal uplift and wound closure effects, and significantly accelerated wound healing with accelerating re-epithelization and ECM remodeling post-ESD. The two-component in-situ hydrogels exhibited great potential in gastrointestinal tract ESD. [Display omitted] •A multifunctional two-component in-situ hydrogel was developed.•The hydrogel can function as submucosal fluid cushion and seal the wound after esophageal ESD.•The hydrogel has greatly facilitated postoperative wound healing in a canine model for esophageal ESD.