Pan-genome inversion index reveals evolutionary insights into the subpopulation structure of Asian rice

• Published in: Nature communications Vol. 14; no. 1; pp. 1567 - 14
• Main Authors: Zhou, Yong, Yu, Zhichao, Chebotarov, Dmytro, Chougule, Kapeel, Lu, Zhenyuan, Rivera, Luis F., Kathiresan, Nagarajan, Al-Bader, Noor, Mohammed, Nahed, Alsantely, Aseel, Mussurova, Saule, Santos, João, Thimma, Manjula, Troukhan, Maxim, Fornasiero, Alice, Green, Carl D., Copetti, Dario, Kudrna, David, Llaca, Victor, Lorieux, Mathias, Zuccolo, Andrea, Ware, Doreen, McNally, Kenneth, Zhang, Jianwei, Wing, Rod A.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.03.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 38; 45; 45/23; 45/91; 631/181/2474; 631/208/212/748; 631/208/8; 631/449/2491; Biological Evolution; Crop production; Cultivated plants; Evolution; Gene expression; Genetic diversity; Genome, Plant - genetics; Genomes; Humanities and Social Sciences; Inversions; Linkage disequilibrium; multidisciplinary; Nucleotide sequence; Oryza - genetics; Oryza rufipogon; Phylogeny; Recombination; Rice; Science; Science (multidisciplinary); Sequence Analysis, DNA
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-023-37004-y

Understanding and exploiting genetic diversity is a key factor for the productive and stable production of rice. Here, we utilize 73 high-quality genomes that encompass the subpopulation structure of Asian rice ( Oryza sativa ), plus the genomes of two wild relatives ( O. rufipogon and O. punctata ), to build a pan-genome inversion index of 1769 non-redundant inversions that span an average of ~29% of the O. sativa cv. Nipponbare reference genome sequence. Using this index, we estimate an inversion rate of ~700 inversions per million years in Asian rice, which is 16 to 50 times higher than previously estimated for plants. Detailed analyses of these inversions show evidence of their effects on gene expression, recombination rate, and linkage disequilibrium. Our study uncovers the prevalence and scale of large inversions (≥100 bp) across the pan-genome of Asian rice and hints at their largely unexplored role in functional biology and crop performance. Pan-genomes provide useful resources for evolutionary studies, functional genomics and breeding of cultivated plants. Here, the authors report a new rice pan-genome including 73 Asian rice and two wild relatives ( Oryza rufipogon and O. punctata ), and reveal the prevalence and scale of large inversions across the pan-genome.