Simultaneous determination of trichothecene mycotoxins in cereals by LC-MS/MS

• Published in: Food science and biotechnology Vol. 31; no. 2; pp. 165 - 174
• Main Authors: Kim, Dan-Bi, Jung, Young Sung, Nam, Tae Gyu, Lee, Sanghee, Yoo, Miyoung
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.02.2022; Springer Nature B.V; 한국식품과학회
• Subjects: Acetonitrile; beans; biotechnology; Cereals; Chemistry; Chemistry and Materials Science; Contamination; Deoxynivalenol; Food Science; Formic acid; HT-2 toxin; markets; Mycotoxins; Nutrition; Research Article; rice; risk; Risk assessment; Silica; Silicon dioxide; Solid phases; solvents; Toxins; 식품과학; Hazard index; Deoxynivalenol; Method validation; Risk assessment; Dispersive-solid phase; Fusarium
• ISSN: 1226-7708; 2092-6456; 2092-6456
• DOI: 10.1007/s10068-021-01024-5

This study was designed to determine the residual trichothecene mycotoxins in cereal samples. The optimal solvent for extraction was 84% (v/v) aqueous acetonitrile with 1% (v/v) formic acid. The best performing clean-up method was dispersive-solid phase with a mixture octadecyl silica and primary-secondary amine. The recoveries for the studied mycotoxins ranged from 83.3 to 92.8%. The methodology was successfully applied for monitoring 100 cereal samples obtained from a Korean market. The bean sample were found to be co-contamination with deoxynivalenol and HT-2 toxin. Deoxynivalenol possessed the highest detection freauency (4/100) and amount (727.38 µg/kg) among the trichothecene mycotoxins. The hazard index was less than 1.0 for all the observed mycotoxins in all cereal samples except one white rice sample (1.2681). This results indicated that periodic risk assessments of trichothecene mycotoxin through cereal intake are necessary for the health and safety.