Mechanical mapping of mammalian follicle development using Brillouin microscopy

• Published in: Communications biology Vol. 4; no. 1; pp. 1133 - 10
• Main Authors: Chan, Chii Jou, Bevilacqua, Carlo, Prevedel, Robert
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 27.09.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 14; 14/63; 631/136/2434/1706; 631/1647/2204; 631/1647/245; 631/57; Animals; Biology; Biomechanical Phenomena; Biomedical and Life Sciences; Cell differentiation; Connective tissues; Cytoplasm; Developmental stages; Extracellular matrix; Female; Follicles; Folliculogenesis; Gene mapping; Infertility; Life Sciences; Mechanical properties; Mechanotransduction; Mechanotransduction, Cellular; Mice - embryology; Mice - growth & development; Microscopy; Morphogenesis; Oocytes; Ovarian Follicle - embryology; Ovarian Follicle - growth & development; Ovaries; Stroma
• ISSN: 2399-3642; 2399-3642
• DOI: 10.1038/s42003-021-02662-5

In early mammalian development, the maturation of follicles containing the immature oocytes is an important biological process as the functional oocytes provide the bulk genetic and cytoplasmic materials for successful reproduction. Despite recent work demonstrating the regulatory role of mechanical stress in oocyte growth, quantitative studies of ovarian mechanical properties remain lacking both in vivo and ex vivo. In this work, we quantify the material properties of ooplasm, follicles and connective tissues in intact mouse ovaries at distinct stages of follicle development using Brillouin microscopy, a non-invasive tool to probe mechanics in three-dimensional (3D) tissues. We find that the ovarian cortex and its interior stroma have distinct material properties associated with extracellular matrix deposition, and that intra-follicular mechanical compartments emerge during follicle maturation. Our work provides an alternative approach to study the role of mechanics in follicle morphogenesis and might pave the way for future understanding of mechanotransduction in reproductive biology, with potential implications for infertility diagnosis and treatment. Chan et al. apply Brillouin microscopy to study changes in tissue material properties during mammalian follicle development. Focusing on the Brillouin loss tangent which is independent of the refractive index and mass density, they find that mammalian folliculogenesis is characterised by region-specific changes in tissue micro-viscosity driven by cell differentiation and extracellular matrix remodelling.