Mechanoporation enables rapid and efficient radiolabeling of stem cells for PET imaging

• Published in: Scientific reports Vol. 12; no. 1; pp. 2955 - 10
• Main Authors: Jung, Kyung Oh, Theruvath, Ashok Joseph, Nejadnik, Hossein, Liu, Anna, Xing, Lei, Sulchek, Todd, Daldrup-Link, Heike E., Pratx, Guillem
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 22.02.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/337; 631/45; 631/532; 631/61; 631/80; 631/92; Adipose tissue; Adipose Tissue - metabolism; Animals; Biocompatibility; Cartilage; Channel pores; Gallium Radioisotopes - pharmacology; Humanities and Social Sciences; Immunotherapy; Ionizing radiation; Knee; Labeling; Microfluidics; multidisciplinary; Nanoparticles; Positron emission tomography; Radiopharmaceuticals - pharmacology; Regenerative medicine; Science; Science (multidisciplinary); Stem cell transplantation; Stem cells; Stem Cells - metabolism; Surgery; Swine; Swine, Miniature; Transplants & implants
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-06938-6

Regenerative medicine uses the patient own stem cells to regenerate damaged tissues. Molecular imaging techniques are commonly used to image the transplanted cells, either right after surgery or at a later time. However, few techniques are fast or straightforward enough to label cells intraoperatively. Adipose tissue-derived stem cells (ADSCs) were harvested from knee joints of minipigs. The cells were labeled with PET contrast agent by flowing mechanoporation using a microfluidic device. While flowing through a series of microchannels, cells are compressed repeatedly by micro-ridges, which open transient pores in their membranes and induce convective transport, intended to facilitate the transport of 68 Ga-labeled and lipid-coated mesoporous nanoparticles (MSNs) into the cells. This process enables cells to be labeled in a matter of seconds. Cells labeled with this approach were then implanted into cartilage defects, and the implant was imaged using positron emission tomography (PET) post-surgery. The microfluidic device can efficiently label millions of cells with 68 Ga-labeled MSNs in as little as 15 min. The method achieved labeling efficiency greater than 5 Bq/cell on average, comparable to 30 min-long passive co-incubation with 68 Ga-MSNs, but with improved biocompatibility due to the reduced exposure to ionizing radiation. Labeling time could also be accelerated by increasing throughput through more parallel channels. Finally, as a proof of concept, ADSCs were labeled with 68 Ga-MSNs and quantitatively assessed using clinical PET/MR in a mock transplant operation in pig knee joints. MSN-assisted mechanoporation is a rapid, effective and straightforward approach to label cells with 68 Ga. Given its high efficiency, this labeling method can be used to track small cells populations without significant effects on viability. The system is applicable to a variety of cell tracking studies for cancer therapy, regenerative therapy, and immunotherapy.