In vitro Immunomodulatory activity of verbascoside from Nepeta ucrainica L

• Published in: Phytotherapy research Vol. 16; no. 6; pp. 593 - 595
• Main Authors: Akbay, Pinar, Calis, Ihsan, Ündeger, Ülkü, Basaran, Nursen, Basaran, A. Ahmet
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.09.2002; Wiley
• Subjects: 1,5,9‐epi‐deoxyloganic acid; 9-epi-deoxyloganic acid; Adjuvants, Immunologic - blood; Adjuvants, Immunologic - pharmacology; Alkaloids - blood; Alkaloids - chemistry; Alkaloids - pharmacology; Beverages; Biological and medical sciences; chemotaxis; Chemotaxis - drug effects; Chromatography, High Pressure Liquid; General pharmacology; Glucosides - blood; Glucosides - chemistry; Glucosides - isolation & purification; Glucosides - pharmacology; Humans; immunomodulatory activity; Iridoid Glucosides; Iridoids - chemistry; Iridoids - isolation & purification; Iridoids - pharmacology; Kazakhstan; Magnetic Resonance Spectroscopy; Medical sciences; Molecular Structure; NBT; Nepeta; Nepeta ucrainica L; Neutrophils - drug effects; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phenols - blood; Phenols - chemistry; Phenols - pharmacology; Plant Extracts - pharmacology; Respiratory Burst - drug effects; Spectrophotometry, Ultraviolet; verbascoside; Kazakhstan; Asia; Molecular structure; HPLC chromatography; Screening test; Medicinal plant; Spectrometry; Folk medicine; Dicotyledones; Angiospermae; Glycoside; Human; Healthy subject; Pharmacognosy; Antioxidant; Chemotaxis; In vitro; Immunomodulator; Analysis method; Labiatae; Plant origin; Isolation; Above ground plant part; Active ingredient; Spermatophyta; Immunosuppressive agent; Neutrophil; Structural analysis
• ISSN: 0951-418X; 1099-1573
• DOI: 10.1002/ptr.990

Nepeta ucrainica L. is used as a herbal tea in Kazakhistan. Phytochemical investigations of the aerial parts of the plant resulted in the isolation of verbascoside (1) and 1,5,9‐epi‐deoxyloganic acid (2). The structures of the compounds were elucidated by spectral (UV, IR, 1H‐NMR and 13C‐NMR) methods and HPLC analysis. The in vitro immunomodulatory activity of verbascoside was investigated by assessing neutrophil function; chemotaxis and intracellular killing activity. Verbascoside showed an increased chemotactic activity in all doses applied compared with the medium used as a negative control and had a positive effect on respiratory burst of neutrophils, but there was an opposite effect with increasing doses, pointing to a possible suppression of neutrophil intracellular killing activity. Copyright © 2002 John Wiley & Sons, Ltd.