Th2 Differentiation Is Unaffected by Jagged2 Expression on Dendritic Cells

• Published in: The Journal of immunology (1950) Vol. 180; no. 12; pp. 7931 - 7937
• Main Authors: Krawczyk, Connie M, Sun, Jie, Pearce, Edward J
• Format: Journal Article
• Language: English
• Published: England Am Assoc Immnol 15.06.2008
• Subjects: Animals; Antigens, Helminth - physiology; Cell Differentiation - immunology; Dendritic Cells - immunology; Dendritic Cells - metabolism; Down-Regulation - immunology; Gene Expression Regulation - immunology; Jagged-2 Protein; Ligands; Membrane Proteins - biosynthesis; Membrane Proteins - genetics; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Knockout; Mice, Transgenic; Schistosoma mansoni; Schistosoma mansoni - immunology; Th2 Cells - cytology; Th2 Cells - immunology; Th2 Cells - metabolism; Toll-Like Receptors - metabolism; Toll-Like Receptors - physiology; Up-Regulation - immunology
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.180.12.7931

Expression of the Jagged Notch ligands by dendritic cells (DCs) has been suggested to play a role in instructing Th2 responses. Supporting this hypothesis, we found that Jagged2 but not Jagged1 expression, correlates with the ability of DCs to induce Th2 responses. Jagged2 expression is up-regulated in response to the helminth soluble Schistosoma mansoni egg Ag, which conditions DCs to induce Th2 responses, and is markedly down-regulated following exposure to TLR agonists that generally promote Th1 responses. Conversely, Jagged1 expression is markedly induced by TLR ligation. Despite these correlations, suppression of expression of Jagged2 using retrovirally delivered small interfering RNA failed to affect the ability of DCs to induce Th2 cell differentiation either in vitro or in vivo. Moreover, retrovirally induced expression of Jagged2 did not enhance the ability of DCs to induce Th2 cell responses. Our data indicate that Jagged2 expression by DCs is not sufficient or required for Th2 cell differentiation.