Prevotella-to-Bacteroides ratio predicts body weight and fat loss success on 24-week diets varying in macronutrient composition and dietary fiber: results from a post-hoc analysis

• Published in: International Journal of Obesity Vol. 43; no. 1; pp. 149 - 157
• Main Authors: Hjorth, Mads F., Blædel, Trine, Bendtsen, Line Q., Lorenzen, Janne K., Holm, Jacob B., Kiilerich, Pia, Roager, Henrik M., Kristiansen, Karsten, Larsen, Lesli H., Astrup, Arne
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.01.2019; Nature Publishing Group
• Subjects: 45/22; 45/23; 45/77; 631/443/319/1488/393; 692/308/2779/777; Adult; Bacteroides; Bacteroides - physiology; Body fat; Body weight; Body weight loss; Calcium; Carbohydrates; Composition; Correlation coefficient; Correlation coefficients; Dairy products; Diet; Diet, Reducing; Dietary fiber; Dietary Fiber - administration & dosage; Dietary intake; Energy Intake; Epidemiology; Feces - microbiology; Female; Gastrointestinal Microbiome - physiology; Gene sequencing; Health Promotion and Disease Prevention; Humans; Internal Medicine; Male; Medicine; Medicine & Public Health; Metabolic Diseases; Middle Aged; Nutrients - administration & dosage; Overweight; Overweight - diet therapy; Overweight - metabolism; Overweight - microbiology; Pretreatment; Prevotella - physiology; Proteins; Public Health; Randomization; Retrospective Studies; RNA, Ribosomal, 16S; rRNA 16S; Weight loss; Weight Loss - physiology
• ISSN: 0307-0565; 1476-5497; 1476-5497
• DOI: 10.1038/s41366-018-0093-2

Background/objectives Individuals with high pre-treatment bacterial Prevotella -to- Bacteroides ( P/B ) ratio have been reported to lose more body weight on diets high in fiber than subjects with a low P/B ratio. Therefore, the aim of the present study was to examine potential differences in dietary weight loss responses between participants with low and high P/B . Subjects/methods Eighty overweight participants were randomized (52 completed) to a 500 kcal/d energy deficit diet with a macronutrient composition of 30 energy percentage (E%) fat, 52 E% carbohydrate and 18 E% protein either high (≈1500 mg calcium/day) or low ( ≤ 600 mg calcium/day) in dairy products for 24 weeks. Body weight, body fat, and dietary intake (by 7-day dietary records) were determined. Individuals were dichotomized according to their pre-treatment P/B ratio derived from 16S rRNA gene sequencing of collected fecal samples to test the potential modification of dietary effects using linear mixed models. Results Independent of the randomized diets, individuals with high P/B lost 3.8 kg (95%CI, 1.8,5.8; P  < 0.001) more body weight and 3.8 kg (95% CI, 1.1, 6.5; P  = 0.005) more body fat compared to individuals with low P/B . After adjustment for multiple covariates, individuals with high P/B ratio lost 8.3 kg (95% CI, 5.8;10.9, P  < 0.001) more body weight when consuming above compared to below 30 g fiber/10MJ whereas this weight loss was 3.2 kg (95% CI, 0.8;5.5, P  = 0.008) among individuals with low P/B ratio [Mean difference: 5.1 kg (95% CI, 1.7;8.6, P  = 0.003)]. Partial correlation coefficients between fiber intake and weight change was 0.90 ( P  < 0.001) among individuals with high P/B ratio and 0.25 ( P  = 0.29) among individuals with low P/B ratio. Conclusions Individuals with high P/B lost more body weight and body fat compared to individuals with low P/B , confirming that individuals with a high P/B are more susceptible to weight loss on a diet rich in fiber.