Determination of serum neutralizing antibodies reveals important difference in quality of antibodies against pertussis toxin in children after infection

• Published in: Vaccine Vol. 39; no. 13; pp. 1826 - 1830
• Main Authors: Zhang, Yuxiao, Li, Yarong, Chen, Zhiyun, Liu, Xiaoguai, Peng, Xiaokang, He, Qiushui
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 26.03.2021; Elsevier Limited
• Subjects: Age; Allergy and Immunology; Anti-PT IgG antibodies; Antibodies; blood serum; Cell culture; Children; China; Cricetulus griseus; Enzyme-linked immunosorbent assay; Immunoglobulin G; Infections; Laboratories; Neutralizing; Ovaries; Pertussis; Pertussis toxin; Pertussis toxin neutralizing antibodies; Serology; Toxins; Vaccination; Vaccines; Whooping cough; United States > US; China; Pertussis; Anti-PT IgG antibodies; Pertussis toxin neutralizing antibodies; Children; China
• ISSN: 0264-410X; 1873-2518; 1873-2518
• DOI: 10.1016/j.vaccine.2021.02.045

•A CHO cell assay was developed to measure PT neutralizing antibodies (PTNAs).•A correlation between serum titers of PTNAs and anti-PT IgG levels was observed.•Patients with same level of anti-PT IgG antibodies can have different PTNAs.•Determination of PTNAs is important for assessment of functional antibodies. To determine neutralizing antibodies to pertussis toxin (PTNAs) in children with suspected pertussis and to compare results of PTNAs and anti-PT IgG antibodies. 172 hospitalized children with suspected pertussis were included. Pertussis was confirmed by culture, PCR and/or serology. PTNAs were determined by Chinese hamster ovary (CHO) cell assay. A correlation between titers of PTNAs and anti-PT IgG levels was noticed in 172 patients (Spearman R = 0.68, P < 0.001). Subjects with same concentrations of anti-PT IgG antibodies could have different titers of PTNAs and the maximum difference observed reached to 1024 times in ELISA-confirmed patients. Moreover, subjects with same titers of PTNAs could have different concentrations of anti-PT IgG antibodies. Our results indicated that in some children high concentrations of anti-PT IgG antibodies do not always mean effective PTNAs induced after infection, stressing the importance of detecting PTNAs after infection and vaccination. Clinical trial registry: Not applicable.