Induction and expression of seed‐specific promoters in Arabidopsis embryo‐defective mutants

• Published in: The Plant journal : for cell and molecular biology Vol. 9; no. 2; pp. 205 - 215
• Main Authors: Devic, Martine, Albert, Sylvie, Delseny, Michel
• Format: Journal Article
• Language: English
• Published: Osney Mead, Oxford OX2 0EL, UK Blackwell Science Ltd 01.02.1996; Blackwell Science
• Subjects: 2S Albumins, Plant; abscisic acid; Arabidopsis; Arabidopsis - embryology; Arabidopsis - genetics; Arabidopsis thaliana; beta-glucuronidase; Biological and medical sciences; Crosses, Genetic; developmental stages; embryo (plant); embryogenesis; embryology; enzyme activity; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Regulation, Plant; Genes, Reporter; genetic variation; genetics; mutants; Mutation; Plant growth. Development of the storage organs; Plant physiology and development; Plant Proteins; Plant Proteins - genetics; promoter regions; Promoter Regions, Genetic; reporter genes; seed development; Seeds; Seeds - embryology; Seeds - genetics; Time Factors; Vegetative apparatus, growth and morphogenesis. Senescence; Morphogenesis; Embryonic development; Embryo; Cruciferae; Arabidopsis; Dicotyledones; Angiospermae; Spermatophyta; Hybridization; Mutation; Gene expression; Histochemistry
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1046/j.1365-313X.1996.09020205.x

In order to assess the importance of morphogenesis on the induction of promoter markers for storage and Lea programmes, advantage was taken of the emb mutations producing embryos arrested at a wide range of developmental stages in Arabidopsis. These embryos are viable during their stage of developmental arrest and continue to divide further, but apparently without further differentiation into the main organs and tissues of the normal embryos. Eight independent emb mutants arrested in their development prior to the cotyledon stage were selected. These emb embryos lack the normal morphology of the wild‐type embryos when the synthesis of storage and Lea proteins are normally initiated. The 2S1‐uidA chimeric gene, representative of the maturation programme and the Em 1‐uidA chimeric gene, representative of the desiccation programme were introduced by crosses into the emb background. In the eight emb lines, the expression of the GUS reporter gene directed by the 2S1 and Em 1 promoters was observed in the aborted seeds irrespective of their stage of developmental arrest. The time of induction of the expression of both promoters was the same in the arrested embryos as compared with the normal embryos within the same silique. Thus, the activation of these two promoters is triggered by the same signal and can occur in the absence of morphogenesis. However, in the absence of normal organ formation, the expression of the reporter gene under the control of the 2S1 and Em 1 promoters was evident throughout the whole seed tissues. In normal seed development, the hormone abscisic acid (ABA) activates the promoters of the 2S1 and Em 1 genes. One of the important members of the signal transduction pathway of ABA is the ABI3 protein. It has been shown previously that this protein is a prerequisite for the induction of Em 1 by ABA in seeds. A good correlation with the expression of the ABI3 promoter and the 2S1 and Em 1 promoters was found in emb seeds tissues. This observation suggests that the promoters of the 2S1 and the Em 1 genes are expressed in the mutant seeds not at a basal level, but are probably induced by ABA, as in normal seed development.