A BAC-Based Physical Map of the Major Autosomes of Drosophila melanogaster

• Published in: Science (American Association for the Advancement of Science) Vol. 287; no. 5461; pp. 2271 - 2274
• Main Authors: Hoskins, Roger A., Nelson, Catherine R., Berman, Benjamin P., Laverty, Todd R., George, Reed A., Ciesiolka, Lisa, Naeemuddin, Mohammed, Arenson, Andrew D., Durbin, James, David, Robert G., Tabor, Paul E., Bailey, Michael R., DeShazo, Denise R., Catanese, Joseph, Mammoser, Aaron, Osoegawa, Kazutoyo, de Jong, Pieter J., Celniker, Susan E., Gibbs, Richard A., Rubin, Gerald M., Scherer, Steven E.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 24.03.2000; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Algorithms; Animals; autosomes; Bacteria; bacterial artificial chromosomes; Biological and medical sciences; Centromere; Centromere - genetics; Chromatin; Chromatin - genetics; chromosome 2; chromosome 3; chromosome mapping; Chromosomes; Chromosomes, Bacterial; Chromosomes, Bacterial - genetics; Classical genetics, quantitative genetics, hybrids; Cloning, Molecular; Coding; Contig Mapping; cytogenetic analysis; DNA Fingerprinting; Drosophila; Drosophila melanogaster; Drosophila melanogaster - genetics; Euchromatin; False positive errors; Fundamental and applied biological sciences. Psychology; Gene Library; Genes, Insect; Genetic Markers; Genetic Vectors; genetics; Genetics of eukaryotes. Biological and molecular evolution; Genome; Genomes; Genomics; In Situ Hybridization; Insects; Invertebrata; Libraries; Map libraries; Molecular Biology; Polytene chromosomes; Repetitive Sequences, Nucleic Acid; Restriction Mapping; Science and Society; Sequence Analysis, DNA; Sequence Tagged Sites; Telomere; Telomere - genetics; Tiling; Genetic mapping; Contig; Arthropoda; Insecta; Invertebrata; Chromosome 3; Chromosome 2; Artificial chromosome; Drosophila melanogaster; Diptera; Physical map; Drosophilidae
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.287.5461.2271

We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.