Single-nucleotide polymorphisms in the IL-4 and IL-13 promoter region in aggressive periodontitis

• Published in: Journal of clinical periodontology Vol. 34; no. 6; pp. 473 - 479
• Main Authors: Gonzales, J. R., Mann, M., Stelzig, J., Bödeker, R. H., Meyle, J.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.06.2007; Blackwell
• Subjects: Adenine; Adult; aggressive periodontitis; Biological and medical sciences; Case-Control Studies; Cytosine; DNA - genetics; Female; Gene Frequency - genetics; General aspects; genetic polymorphisms; Genotype; Gingival Hemorrhage - genetics; Gingival Hemorrhage - immunology; Homozygote; Humans; interleukin-13; Interleukin-13 - genetics; interleukin-4; Interleukin-4 - genetics; Male; Medical sciences; Periodontal Attachment Loss - genetics; Periodontal Attachment Loss - immunology; Periodontal Pocket - genetics; Periodontal Pocket - immunology; Periodontitis - genetics; Periodontitis - immunology; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide - genetics; Promoter Regions, Genetic - genetics; risk factors; Single-Blind Method; Thymine; Human; interleukin-4; risk factors; Stomatology; Interleukin 13; Dentistry; genetic polymorphisms; interleukin-13; Promoter; Periodontal disease; Interleukin 4; Periodontitis; Risk factor; Nucleotide; Genetics; aggressive periodontitis; Polymorphism
• ISSN: 0303-6979; 1600-051X
• DOI: 10.1111/j.1600-051X.2007.01086.x

Introduction: IL‐4 and IL‐13 polymorphisms have been shown to influence the susceptibility to systemic diseases. In this study, possible associations between the IL‐4 −590 C→T, IL‐4 −34 C→T, IL‐13 −1112 C→T and IL‐13 −1512 A→C promoter polymorphisms were investigated in subjects with generalized aggressive periodontitis (AgP) compared with healthy individuals. Material and Methods: Fifty‐eight patients with diagnosis of generalized AgP and 51 matched healthy controls participated in the study. Blood samples were collected and DNA isolated. Molecular analyses were performed by PCR‐RFLP in a blind fashion. Genotype and allele frequencies among study groups were compared using Fisher's exact test (α value: 0.05). Pearson's χ2 test was used for analysis of Hardy–Weinberg equilibrium. Results: The frequency of the IL‐4 −590 T/T and IL‐4 −34 T/T genotypes differed significantly between groups (p=0.05, 0.02, respectively), although the allele frequencies were similar. There was a higher frequency of the IL‐4 −590 T/T and IL‐4 −34 T/T genotypes in patients with AgP compared with controls. The genotype and allele frequencies of the IL‐13 polymorphisms did not differ between groups. Conclusions: This study demonstrated an association between the IL‐4 −590 T/T and IL‐4 −34 T/T genotypes and AgP. Further research is necessary to prove if there is an association of these polymorphisms with AgP, and if the polymorphisms have a functional effect.