A mitogen-activated protein kinase NtMPK4 activated by SIPKK is required for jasmonic acid signaling and involved in ozone tolerance via stomatal movement in tobacco [Nicotiana]
The mitogen-activated protein kinase (MAPK) cascade is involved in responses to biotic and abiotic stress in plants. In this study, we isolated a new MAPK, NtMPK4, which is a tobacco homolog of Arabidopsis MPK4 (AtMPK4). NtMPK4 was activated by wounding along with two other wound-responsive tobacco...
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Published in | Plant and cell physiology Vol. 46; no. 12; pp. 1902 - 1914 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Japan
Oxford University Press
01.12.2005
Oxford Publishing Limited (England) |
Subjects | |
Online Access | Get full text |
ISSN | 0032-0781 1471-9053 |
DOI | 10.1093/pcp/pci211 |
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Summary: | The mitogen-activated protein kinase (MAPK) cascade is involved in responses to biotic and abiotic stress in plants. In this study, we isolated a new MAPK, NtMPK4, which is a tobacco homolog of Arabidopsis MPK4 (AtMPK4). NtMPK4 was activated by wounding along with two other wound-responsive tobacco MAPKs, WIPK and SIPK. We found that NtMPK4 was activated by salicylic acid-induced protein kinase kinase (SIPKK), which has been isolated as an SIPK-interacting MAPK kinase. In NtMPK4 activity-suppressed tobacco, wound-induced expression of jasmonic acid (JA)-responsive genes was inhibited. NtMPK4-silenced plants showed enhanced sensitivity to ozone. Inversely, transgenic tobacco plants, in which SIPKK or the constitutively active type SIPKKEE was overexpressed, exhibited greater responsiveness to wounding with enhanced resistance to ozone. We further found that NtMPK4 was expressed preferentially in epidermis, and the enhanced sensitivity to ozone in NtMPK4-silenced plants was caused by an abnormal regulation of stomatal closure in an ABA-independent manner. These results suggest that NtMPK4 is involved in JA signaling and in stomatal movement. |
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Bibliography: | 2006005004 H50 local:pci211 Corresponding author: E-mail, sseo71@affrc.go.jp; Fax, +81-29-838-7469. istex:0B377828F48E144E12C80CCD76AFB8DDB2B85B7E ark:/67375/HXZ-NP34F9TX-Z ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
ISSN: | 0032-0781 1471-9053 |
DOI: | 10.1093/pcp/pci211 |