Duck Pancreatic Acinar Cell as a Unique Model for Independent Cholinergic Stimulation-Secretion Coupling

• Published in: Cellular and molecular neurobiology Vol. 29; no. 5; pp. 747 - 756
• Main Authors: Wang, Bi Jue, Liang, Hui Yuan, Cui, Zong Jie
• Format: Journal Article
• Language: English
• Published: Boston Boston : Springer US 01.07.2009; Springer US
• Subjects: Acetylcholine - metabolism; Acetylcholine - pharmacology; Amylases - metabolism; Animals; Biomedical and Life Sciences; Biomedicine; Calcium Signaling - drug effects; Cell Biology; Cell Separation; Colforsin - pharmacology; Ducks - metabolism; Models, Biological; Neurobiology; Neurosciences; Original Paper; Pancreas, Exocrine - cytology; Pancreas, Exocrine - drug effects; Pancreas, Exocrine - metabolism; Pituitary Adenylate Cyclase-Activating Polypeptide - pharmacology; Rats; Receptors, Cholinergic - metabolism; Stimulus-secretion coupling; Duck pancreatic acinar cell; PACAP; Muscarinic receptors
• ISSN: 0272-4340; 1573-6830; 1573-6830
• DOI: 10.1007/s10571-009-9400-8

This paper investigated the role of acetylcholine (ACh) in physiological regulation of amylase secretion in avian exocrine pancreas. In the isolated duck pancreatic acini, ACh dose dependently stimulated amylase secretion, with a maximal effective concentration at 10 μM. The cAMP-mobilizing compounds forskolin, vasoactive intestinal peptide (VIP)/pituitary adenylate cyclase activating peptide (PACAP) receptor (VPAC) agonists PACAP-38 and PACAP-27 had no effect on the dose-response curve. ACh dose dependently induced increases in cytosolic Ca²⁺ concentration ([Ca²⁺] c ), with increasing concentrations transforming oscillations into plateau increases. Forskolin (10 μM), PACAP-38 (1 nM), PACAP-27 (1 nM), or VIP (10 nM) alone did not stimulate [Ca²⁺] c increase; neither did they modulate ACh-induced oscillations, nor made ACh low concentration effective. These data indicate that ACh-stimulated zymogen secretion in duck pancreatic acinar cells is not subject to modulation from the cAMP signaling pathway; whereas it has been widely reported in the rodents that ACh-stimulated exocrine pancreatic secretion is significantly enhanced by cAMP-mobilizing agents. This makes the duck exocrine pancreas unique in that cholinergic stimulus-secretion coupling is not subject to cAMP regulation.