Carbapenemases in Gram-Negative Bacteria : Laboratory Detection and Clinical Significance

• Published in: BioMed research international Vol. 2014; no. 2014; pp. 1 - 3
• Main Authors: Bedenic, Branka, Plecko, Vanda, Sardelic, Sanda, Uzunovic, Selma, Torkar, Karmen Godic
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2014; Hindawi Publishing Corporation; John Wiley & Sons, Inc
• Subjects: Anti-Bacterial Agents - therapeutic use; Antibiotics; Bacteria; Bacterial proteins; Bacterial Proteins - genetics; Bacterial Proteins - isolation & purification; Bacteriology; beta-Lactamases - genetics; beta-Lactamases - isolation & purification; Carbapenems; Drug resistance in microorganisms; Drug Resistance, Bacterial - genetics; E coli; Enzymes; Genetic aspects; Gram-negative bacteria; Gram-Negative Bacteria - drug effects; Gram-Negative Bacteria - enzymology; Gram-Negative Bacteria - genetics; Humans; Infections; Laboratories; Microbiology; Microorganisms; Mortality; Plasmids; Properties; Surveillance; Greece
• ISSN: 2314-6133; 2314-6141; 2314-6141
• DOI: 10.1155/2014/841951

[...]simplex or multiplex PCR, real-time PCR, or hybridization tests could significantly improve detection of carbapenemase genes in clinical laboratory bypassing the sensitivity and specificity problems with phenotypic tests. [...]facing the global crisis in antibiotic resistance, presented by rapid dissemination of carbapenemase producing Gram-negative bacteria, many issues remain controversial, especially detection methods and treatment options.