Production of Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) by Human Astrocytic Tumors

• Published in: Neurologia medico-chirurgica Vol. 35; no. 10; pp. 728 - 731
• Main Authors: ARAI, Yoshikazu, SATO, Kazufumi, KODERA, Toshiaki, NAKAGAWA, Takao, KABUTO, Masanori, KUBOTA, Toshihiko
• Format: Journal Article
• Language: English
• Published: Japan The Japan Neurosurgical Society 1995; 日本脳神経外科学会
• Subjects: Adult; Aged; Antibodies, Monoclonal - pharmacology; astrocytic tumor; Astrocytoma - pathology; Astrocytoma - ultrastructure; Brain - pathology; Brain - ultrastructure; Brain Neoplasms - pathology; Brain Neoplasms - ultrastructure; Cell Movement - drug effects; Culture Techniques; enzyme immunoassay; Female; Humans; Immunoenzyme Techniques; Immunohistochemistry; Male; Metalloendopeptidases - metabolism; Middle Aged; Neoplasm Invasiveness; Sialoglycoproteins - metabolism; tissue inhibitor of metalloproteinases-1; tumor invasion
• ISSN: 0470-8105; 1349-8029
• DOI: 10.2176/nmc.35.728

Production of tissue inhibitor of metalloproteinases-1 (TIMP-1), a specific inhibitor of matrix metalloproteinases, was investigated in human astrocytic tumors (n = 15) and normal brain tissues (n = 3). Enzyme immunoassay indicated that TIMP-1 levels in the culture media of tumor explants were significantly higher in glioblastomas as compared with anaplastic astrocytomas, astrocytomas, and normal brain tissues. Immunohistochemistry using a monoclonal antibody against TIMP-1 demonstrated that TIMP-1 was detectable mainly in tumor cells, especially in glioblastoma. These results suggest that increased expression of TIMP-1 is associated with the malignant progression of astrocytic tumors.