AUTO-ANTIBODY AGAINST ADENINE NUCLEOTIDE TRANSLOCATOR IN DILATED CARDIOMYOPATHY AND MYOCARDITIS : Incidence and Relation to Cardiac Function and Morphology

• Published in: JAPANESE CIRCULATION JOURNAL Vol. 57; no. 12; pp. 1150 - 1158
• Main Authors: TAKEMOTO, MASAO, KUSACHI, SHOZO, URABE, NORIO, TSUJI, TAKAO, INOUE, KIMIHITO
• Format: Journal Article
• Language: English
• Published: Kyoto The Japanese Circulation Society 1993; Japanese Circulation Society; 社団法人日本循環器学会
• Subjects: Adult; Aged; ATP-ADP carrier protein; Autoantibodies - immunology; Autoantibodies - isolation & purification; Biological and medical sciences; Blotting, Western; Cardiology. Vascular system; Cardiomyopathy, Dilated - immunology; Cardiomyopathy, Dilated - pathology; Cardiomyopathy, Dilated - physiopathology; Echocardiography; Electrophoresis, Polyacrylamide Gel; Enzyme linked immunosorbent assay; Female; Heart; Heart disease; Humans; Male; Medical sciences; Middle Aged; Mitochondria; Mitochondrial ADP, ATP Translocases - immunology; Mitochondrial ADP, ATP Translocases - isolation & purification; Myocarditis - immunology; Myocarditis - pathology; Myocarditis - physiopathology; Myocarditis. Cardiomyopathies; Western blotting; Human; Myocarditis; Translocation; Mitochondria; Congestive hypertrophic cardiomyopathy; Heart disease; Autoantibody; Cardiovascular disease; Exploration; Myocardial disease; Adenine nucleotide
• ISSN: 0047-1828; 1347-4839
• DOI: 10.1253/jcj.57.1150

Using an enzyme-linked immunosorbent assay, we measured anti-adenine nucleotide translocator (ANT) antibody in control subjects and in patients with dilated cardiomyopathy (DCM), myocarditis, and other heart diseases. Analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blotting, and immunoabsorption tests confirmed accurate purification of the ANT protein and correct measurememt of anti-ANT auto-antibody. Anti-ANT anti-body was detected in 6 of 37 cases (16%) of DCM and in 5 of 12 cases (42%) of myocarditis. This antibody was not positive in other heart diseases or in apparently healthy controls. There were no differences between anti-ANT autoanti-body-positive and -negative DCM or myocarditis patients in any of the cardiac parameters examined (left ventricular ejection fraction, LVEF; left ventricular end-diastolic dimension, LVDd; and cardiothoracic ratio, CTR). Moreover, in patients who were positive for anti-ANT auto-antibody, no positive correlations were found between the parameters and anti-ANT antibody titers in either DCM or myocarditis. Although these results indicate that the detection of anti-ANT antibody was achieved at a high specificity and could have certain diagnostic value in DCM and myocarditis, there was no statistically significant relationship between the cardiac parameters (LVEF, LVDd, and CTR) and anti-ANT antibody titers in either DCM or myocarditis. Some compensatory mechanism of ventricular function may mask the effects of the anti-ANT auto-antibody or alternatively, this auto-antibody may have mimic effects on the pathogenesis and/or progression of DCM and myocarditis.