Identification and characterization of murine DNAM-1 (CD226) and its poliovirus receptor family ligands

• Published in: Biochemical and biophysical research communications Vol. 329; no. 3; pp. 996 - 1000
• Main Authors: Tahara-Hanaoka, Satoko, Miyamoto, Akitomo, Hara, Ayumi, Honda, Shin-ichiro, Shibuya, Kazuko, Shibuya, Akira
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.04.2005; ACADEMIC PRESS INC ELSEVIER SCIENCE
• Subjects: Amino Acid Sequence; Animals; Antigens, Differentiation, T-Lymphocyte - chemistry; Antigens, Differentiation, T-Lymphocyte - immunology; Binding Sites; CD226; Cells, Cultured; Costimulatory molecule; DNAM-1; Humans; Ligands; Membrane Proteins - chemistry; Membrane Proteins - immunology; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Nectin-2; NK cells; Poliovirus; Poliovirus receptor; Protein Binding; Protein Interaction Mapping; PRR-2; Receptors, Virus - chemistry; Receptors, Virus - immunology; Sequence Homology, Amino Acid; T cells; T-Lymphocytes - immunology; NK cells; Nectin-2; Poliovirus receptor; PRR-2; DNAM-1; CD226; T cells; Costimulatory molecule
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2005.02.067

The leukocyte adhesion molecule DNAM-1 (CD226) is a member of the immunoglobulin superfamily and constitutively expressed on the majority of CD4+ and CD8+ T lymphocytes, natural killer (NK) cells, monocytes/macrophages, and a subset of B lymphocytes. The poliovirus receptor (PVR; CD155) and its family member nectin 2 (CD112) have recently been identified as the ligands for DNAM-1. Interaction of DNAM-1 with the ligands induces NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. However, in vivo function of the receptor–ligand interaction has remained unclear. Here, we identified murine DNAM-1 and PVR homologues that physically and functionally bind each other. We demonstrated that ligand binding of murine DNAM-1 induced a costimulatory signal in antigen-specific CD8+ T cells. These results should provide a useful animal model to explore a role of DNAM-1 in immune responses in vivo.