The regulation of maturation promoting factor during prophase I arrest and meiotic entry in mammalian oocytes

• Published in: Molecular and cellular endocrinology Vol. 382; no. 1; pp. 480 - 487
• Main Authors: Adhikari, Deepak, Liu, Kui
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 25.01.2014
• Subjects: Activation; Animals; Arrays; Cell Cycle Checkpoints; Cellular; cyclins; Degradation; dephosphorylation; Endocrinology and Diabetes; Endokrinologi och diabetes; GERMINAL VESICLE BREAKDOWN; GREATWALL KINASE; Humans; LUTEINIZING-HORMONE; M-PHASE ENTRY; Maintenance; mammals; Mammals - metabolism; Maturation; maturation-promoting factor; Maturation-Promoting Factor - metabolism; meiosis; Meiosis resumption; Meiotic Prophase I; MOUSE OOCYTES; MPF; OKADAIC ACID; Oocyte; oocytes; Oocytes - cytology; Oocytes - metabolism; ovulation; Phosphoprotein Phosphatases - metabolism; phosphoproteins; PHOSPHORYLATION SITES; PORCINE GROWING OOCYTES; prophase; Protein phosphatases; protein subunits; PROTEIN-KINASE-A; puberty; Reentry; signal transduction; Surges; TO-CELL COMMUNICATION; Meiosis resumption; Protein phosphatases; MPF; Oocyte
• ISSN: 0303-7207; 1872-8057; 1872-8057
• DOI: 10.1016/j.mce.2013.07.027

•The process of oocyte meiotic maturation is lengthy, complex, and discontinuous.•Low MPF activity maintains prophase I arrest in oocytes.•Low MPF activity is maintained by CDK1 inhibition and cyclin B1 degradation.•Meiosis resumption needs CDK1 activation and protein phosphatase (PP) inhibition.•PP inhibition maintains phosphorylation of CDK1 substrates. Mammalian oocytes arrest at prophase of meiosis I at around birth and they remain arrested at this stage until puberty when the preovulatory surge of luteinizing hormone (LH) causes ovulation. Prophase I arrest in the immature oocyte results from the maintenance of low activity of maturation promoting factor (MPF), which consists of a catalytic subunit (CDK1) and regulatory subunit (cyclin B1). Phosphorylation-mediated inactivation of CDK1 and constant degradation of cyclin B1 keep MPF activity low during prophase I arrest. LH-mediated signaling manipulates a vast array of molecules to activate CDK1. Active CDK1 not only phosphorylates different meiotic phosphoproteins during the resumption of meiosis but also inhibits their rapid dephosphorylation by inhibiting the activities of CDK1 antagonizing protein phosphatases (PPs). In this way, CDK1 both phosphorylates its substrates and protects them from being dephosphorylated. Accumulating evidence suggests thatthe net MPF activity that drives the resumption of meiosis in oocytes depends on the activation status of CDK1 antagonizing PPs. This review aims to provide a summary of the current understanding of the signaling pathways involved in regulating MPF activity during prophase I arrest and reentry into meiosis of mammalian oocytes.