The Secretome of Human Deciduous Tooth-Derived Mesenchymal Stem Cells Enhances In Vitro Wound Healing and Modulates Inflammation

• Published in: Pharmaceutics Vol. 17; no. 8; p. 961
• Main Authors: Payão, Thais Simião, Pellegrini, Vanessa, Morari, Joseane, Gonçalves, Gisele Mara Silva, de Godoy, Maria Carolina Ximenes, Gambero, Alessandra, Reis, Leonardo O., Velloso, Lício Augusto, Araújo, Eliana Pereira, Pascoal, Lívia Bitencourt
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.07.2025; MDPI
• Subjects: Aldehydes; anti-inflammatory effect; Antibiotics; Care and treatment; Cell culture; Dental pulp; Dextrose; Extracellular matrix; Flow cytometry; Genes; Glucose; Health aspects; human dental pulp-derived mesenchymal stem cells; Inflammation; Pediatrics; Pressure ulcers; Regenerative medicine; secretome; Skin; skin regeneration; Stem cells; Transforming growth factors; Vascular endothelial growth factor; Wound healing; Wounds and injuries; Brazil; United States; Massachusetts; Illinois; United States > US; Japan; wound healing; anti-inflammatory effect; secretome; human dental pulp-derived mesenchymal stem cells; skin regeneration
• ISSN: 1999-4923; 1999-4923
• DOI: 10.3390/pharmaceutics17080961

Background/Objectives: Chronic wounds represent a significant clinical and public health challenge due to impaired tissue repair and high associated morbidity. This study investigates the therapeutic potential of the secretome derived from human mesenchymal stem cells obtained from the pulp of deciduous teeth (hDP-MSCs) in promoting skin wound healing. Methods: After confirming the mesenchymal identity and multipotent differentiation potential of hDP-MSCs by using flow cytometry and histological staining, the effects of the secretome on human keratinocyte (HaCaT) cultures were evaluated. Results: Scratch assays, performed under high- and low-glucose conditions, demonstrated that the secretome significantly promoted keratinocyte migration and wound closure without compromising cell viability. Additionally, the secretome modulated the expression of key genes involved in inflammation and tissue regeneration, including IL-1β, TNF-α, TGF-β1, and VEGF-α, in a time-dependent manner. Under inflammatory conditions induced by lipopolysaccharide, co-treatment with the secretome significantly reduced TNF-α expression and increased TGF-β1 expression, suggesting an anti-inflammatory effect. Conclusions: These findings indicate the potential of the hDP-MSC-derived secretome as a promising cell-free therapeutic strategy capable of accelerating skin regeneration and modulating the inflammatory response during the wound healing process.