Analysis of the association between lactotransferrin (LTF) gene polymorphism and dental caries

• Published in: Journal of applied oral science Vol. 18; no. 2; pp. 166 - 170
• Main Authors: Azevedo, Luiza Foltran, Pecharki, Giovana Daniela, Brancher, João Armando, Cordeiro Junior, Carlos Alberto, Medeiros, Kamilla Gabriella dos Santos, Antunes, Alessandra Armstrong, Arruda, Eduardo Silva, Werneck, Renata Iani, Azevedo, Luciana Reis de, Mazur, Rui Fernando, Moysés, Samuel Jorge, Moysés, Simone Tetü, Faucz, Fábio Rueda, Trevilatto, Paula Cristina
• Format: Journal Article
• Language: English
• Published: Brazil Faculdade de Odontologia de Bauru da Universidade de São Paulo 01.04.2010; Faculdade De Odontologia De Bauru - USP; University of São Paulo
• Subjects: Alleles; Amino Acid Substitution; Arginine; Base Sequence; Case-Control Studies; Child; Dental caries; Dental Caries - genetics; Dental Caries Susceptibility - genetics; DENTISTRY, ORAL SURGERY & MEDICINE; DMF Index; DNA Mutational Analysis; Exon 2; Gene Frequency; Gene polymorphism; Humans; Lactoferrin - genetics; LTF; Lysine; Molecular Sequence Data; Original; Polymorphism, Single Nucleotide; Polymorphism, Single-Stranded Conformational; Saliva - metabolism; Dental caries; Exon 2; Gene polymorphism; LTF
• ISSN: 1678-7757; 1678-7765; 1678-7757; 1678-7765
• DOI: 10.1590/S1678-77572010000200011

The present study evaluated the association between lactotransferrin (LTF) gene polymorphism (exon 2, A/G, Lys/Arg) and dental caries. A convenience sample of 110 individuals, 12 years old, was divided into: group 1, 48 individuals without caries experience (DMFT=0), and group 2, 62 subjects with caries experience (DMFT>or=1). DNA was obtained from a mouthwash with 3% glucose solution, followed by a scrapping of the oral mucosa. After DNA purification, polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP) was performed to access the study polymorphism. The LTF A/G (Lys/Arg) polymorphism had been previously reported as located in exon 1. Allele 1 of the study polymorphism was associated with low DMFT index and showed a protective effect against caries experience (OR=0.16, IC=0.03-0.76, p=0.01). Lactotransferrin A/G (exon 2, Lys/Arg) polymorphism was associated with susceptibility to dental caries in 12-year-old students.