TRFs and tiRNAs sequence in acute rejection for vascularized composite allotransplantation

• Published in: Scientific data Vol. 9; no. 1; pp. 544 - 10
• Main Authors: Fang, Yuan, Li, Haibo, Chen, Jingting, Xiong, Yao, Li, Xu, Sun, Binbin, Li, Shengli, Zhou, Jianda, Wang, Shoubao
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 07.09.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/114/2163; 692/53/2423; Bioinformatics; Data Descriptor; Gene expression; Hospitals; Humanities and Social Sciences; Laboratory animals; MAP kinase; multidisciplinary; Nucleotide sequence; Reverse transcription; Science; Science (multidisciplinary); Signal transduction; Surgery; Transcriptomes; Transfer RNA; Transplantation
• ISSN: 2052-4463; 2052-4463
• DOI: 10.1038/s41597-022-01577-y

Illumina tRFs & tiRNAs-seq analysis was used to characterize the whole transcriptomes of acute rejection caused by vascularized composite allotransplantation (VCA). tRFs & tiRNAs-seq information for muscle samples with VCA was obtained and compared with similar information for same age- and sex-matched healthy control subjects. The expression of 16 tRFs and tiRNAs, including 5 up-regulated target genes and 11 down-regulated target genes, were significantly different. According to bioinformatics analysis and reverse transcription quantitative polymerase chain reaction, we speculate that tiRNA-1-34-Glu-CTC-1 plays an important role in VCA-induced acute rejection by regulating the CACNA1D gene in the MAPK signaling pathway The findings provide the whole-transcriptome signatures of acute rejection for VCA, allowing further exploration of gene expression patterns/signatures associated with the various clinical symptoms of acute rejection for VCA. Measurement(s) gene expression Technology Type(s) RNA sequence Factor Type(s) RNA Sample Characteristic - Organism rn6 Sample Characteristic - Location China