Impact of DNA methylation on 3D genome structure

• Published in: Nature communications Vol. 12; no. 1; pp. 3243 - 17
• Main Authors: Buitrago, Diana, Labrador, Mireia, Arcon, Juan Pablo, Lema, Rafael, Flores, Oscar, Esteve-Codina, Anna, Blanc, Julie, Villegas, Nuria, Bellido, David, Gut, Marta, Dans, Pablo D., Heath, Simon C., Gut, Ivo G., Brun Heath, Isabelle, Orozco, Modesto
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.05.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 14/63; 45/15; 45/22; 45/23; 45/44; 45/70; 45/90; 45/91; 631/114/2397; 631/208/176/1988; 631/337/100/102; 631/337/386; 64; Cellular structure; Chromatin; Deoxyribonucleic acid; DNA; DNA methylation; DNA structure; Epigenetics; Eukaryotes; Gene expression; Genomes; Heterochromatin; Humanities and Social Sciences; Mammals; Mimicry; multidisciplinary; Multilayers; Positioning devices (machinery); Science; Science (multidisciplinary); Signal processing; Structure-function relationships; Yeast
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-23142-8

Determining the effect of DNA methylation on chromatin structure and function in higher organisms is challenging due to the extreme complexity of epigenetic regulation. We studied a simpler model system, budding yeast, that lacks DNA methylation machinery making it a perfect model system to study the intrinsic role of DNA methylation in chromatin structure and function. We expressed the murine DNA methyltransferases in Saccharomyces cerevisiae and analyzed the correlation between DNA methylation, nucleosome positioning, gene expression and 3D genome organization. Despite lacking the machinery for positioning and reading methylation marks, induced DNA methylation follows a conserved pattern with low methylation levels at the 5’ end of the gene increasing gradually toward the 3’ end, with concentration of methylated DNA in linkers and nucleosome free regions, and with actively expressed genes showing low and high levels of methylation at transcription start and terminating sites respectively, mimicking the patterns seen in mammals. We also see that DNA methylation increases chromatin condensation in peri-centromeric regions, decreases overall DNA flexibility, and favors the heterochromatin state. Taken together, these results demonstrate that methylation intrinsically modulates chromatin structure and function even in the absence of cellular machinery evolved to recognize and process the methylation signal. Multi-layered epigenetic regulation in higher eukaryotes makes it challenging to disentangle the individual effects of modifications on chromatin structure and function. Here, the authors expressed mammalian DNA methyltransferases in yeast, which have no DNA methylation, to show that methylation has intrinsic effects on chromatin structure.