Aptamer-Conjugated Superparamagnetic Ferroarabinogalactan Nanoparticles for Targeted Magnetodynamic Therapy of Cancer

• Published in: Cancers Vol. 12; no. 1; p. 216
• Main Authors: Kolovskaya, Olga S., Zamay, Tatiana N., Zamay, Galina S., Babkin, Vasily A., Medvedeva, Elena N., Neverova, Nadezhda A., Kirichenko, Andrey K., Zamay, Sergey S., Lapin, Ivan N., Morozov, Evgeny V., Sokolov, Alexey E., Narodov, Andrey A., Fedorov, Dmitri G., Tomilin, Felix N., Zabluda, Vladimir N., Alekhina, Yulia, Lukyanenko, Kirill A., Glazyrin, Yury E., Svetlichnyi, Valery A., Berezovski, Maxim V., Kichkailo, Anna S.
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.01.2020; MDPI
• Subjects: Aptamers; Arabinogalactan; Asialoglycoprotein receptors; Biocompatibility; Cancer therapies; Cell death; drug delivery; Extracellular matrix; Fibronectin; Heat shock proteins; Hydrogen bonds; Internalization; Iron; Lysis; Magnetic fields; Magnetic resonance imaging; magnetically induced cell disruption; magnetodynamic therapy; Nanoparticles; Oligonucleotides; Precision medicine; Simulation; superparamagnetic ferroarabinogalactans; Tumor cells; аптамеры; арабиногалактан; магнитно-резонансная томография; магнитодинамическая терапия; онкология; суперпарамагнитные наночастицы; таргетная терапия; ферроарабиногалактан; magnetic resonance imaging; magnetically induced cell disruption; magnetodynamic therapy; superparamagnetic ferroarabinogalactans; arabinogalactan; drug delivery; aptamers
• ISSN: 2072-6694; 2072-6694
• DOI: 10.3390/cancers12010216

Nanotechnologies involving physical methods of tumor destruction using functional oligonucleotides are promising for targeted cancer therapy. Our study presents magnetodynamic therapy for selective elimination of tumor cells in vivo using DNA aptamer-functionalized magnetic nanoparticles exposed to a low frequency alternating magnetic field. We developed an enhanced targeting approach of cancer cells with aptamers and arabinogalactan. Aptamers to fibronectin (AS-14) and heat shock cognate 71 kDa protein (AS-42) facilitated the delivery of the nanoparticles to Ehrlich carcinoma cells, and arabinogalactan (AG) promoted internalization through asialoglycoprotein receptors. Specific delivery of the aptamer-modified FeAG nanoparticles to the tumor site was confirmed by magnetic resonance imaging (MRI). After the following treatment with a low frequency alternating magnetic field, AS-FeAG caused cancer cell death in vitro and tumor reduction in vivo. Histological analyses showed mechanical disruption of tumor tissues, total necrosis, cell lysis, and disruption of the extracellular matrix. The enhanced targeted magnetic theranostics with the aptamer conjugated superparamagnetic ferroarabinogalactans opens up a new venue for making biocompatible contrasting agents for MRI imaging and performing non-invasive anti-cancer therapies with a deep penetrated magnetic field.